Fig. 8

Experimental design. Female C57BL/6J and NOD/ShiLtJ mice were obtained at 8 weeks old. Mice (n = 9 per group) were exposed four times over the course of 2 weeks, to either DEP (10 µg in 50 ul per dose), silica (1 mg in 50 µl per dose), both, or vehicle only (50 µl). Micro-CT scans were performed 8 weeks after the start of the experiment and on the day of harvest (12 weeks after start experiment). Lung function measurements using FlexiVent were performed on the day of harvest. Mice were sacrificed and harvested in week 12. BAL fluid was collected for differential cell counts, multiplex cytokine ELISA, and assessment of antinuclear antibodies. Serum was collected for assessment of antinuclear antibodies. Lungs were collected for histopathological assessment, based on formalin fixed paraffin embedded (PFFE) H&E and Sirius Red stained slides