Fig. 6

Supplement with Bcl-2 alleviated apoptosis in PS-NPs-exposed human trophoblast cells. (A-C) The mRNA and protein levels of Bcl-2 in Swan 71 cells with Bcl-2 overexpression. (D-G) The protein levels of Cleaved-caspase-2, Caspase-3, and Cleaved-caspase-3 in trophoblast cells exposed to 0 or 100 µg/mL PS-NPs and transfected with or without Bcl-2 overexpression plasmid for 24 h. (H, I) MMP levels in trophoblast cells exposed to 0 or 100 µg/mL PS-NPs and transfected with or without Bcl-2 overexpression plasmid for 1 h (scale bar, 20 μm). (J, K) Flow cytometry analysis and quantification of apoptosis rates (total early and late apoptosis) in trophoblast cells exposed to 0 or 100 µg/mL PS-NPs and transfected with or without Bcl-2 overexpression plasmid for 24 h