Fig. 7

Higher levels of apoptosis and ROS in RM vs. HC villous tissues. (A-B) Representative images of TUNEL analysis of apoptosis levels in HC and RM villous tissues (scale bar, 100 μm) and their relative quantification of the fluorescence intensity (each n = 12). (C-D) Representative images of ROS assays in HC and RM villous tissues (scale bar, 100 μm) and their relative quantification of fluorescence intensity (each n = 12). (E-H) The protein levels of Bcl-2, Cleaved-caspase-2, and Cleaved-caspase-3 in HC and RM villous tissues (each n = 12). (I-K) The correlation analysis between the protein levels of Bcl-2 (I), Cleaved-caspase-2 (J), or Cleaved-caspase-3 (K) and PS fragment content in HC (round) and RM (square) groups (each n = 12)