Fig. 8

PS-NPs exposure increased placental apoptosis and thus induced mouse miscarriage. (A-B) Representative images of TUNEL analysis of apoptosis levels in PS-NPs-exposed mouse placental tissues (scale bar, 100 μm) and their relative quantification of fluorescence intensity (each n = 9). (C-D) Representative images of ROS assays in PS-NPs-exposed mouse placental tissues (scale bar, 100 μm) and their relative quantification of fluorescence intensity (each n = 9). (E-H) The protein levels of murine Bcl-2, Cleaved-caspase-2, and Cleaved-caspase-3 in placental tissues of PS-NPs-exposed mice (each n = 6). (I) Schematic diagram of mouse intervention assays. (J-K) The embryo adsorption and miscarriage rates in pregnant mice treated with PS-NPs + Vector or PS-NPs + OE-Bcl-2 (each n = 9, scale bar, 1 cm). (L-M) Representative images of TUNEL analysis of apoptosis levels in placental tissues of PS-NPs-exposed mice treated with PS-NPs + Vector or PS-NPs + OE-Bcl-2 (scale bar, 100 μm) and their relative quantification of fluorescence intensity (each n = 9). (N-O) Representative images of ROS assays in placental tissues of PS-NPs-exposed mice treated with PS-NPs + Vector or PS-NPs + OE-Bcl-2 (scale bar, 100 μm) and their relative quantification of fluorescence intensity (each n = 9). (P, Q) The protein levels of Bcl-2, Cleaved-caspase-2, and Cleaved-caspase-3 in placental tissues of mice treated with PS-NPs + Vector or PS-NPs + OE-Bcl-2 (each n = 6)