Fig. 2

Oxidative stress in pHBEC-ALI exposed to woodsmoke. Prior to the study, pHBEC-ALI expressing Grx1-roGFP2 were deprived of glucose for 2 h. Woodsmoke or filtered room air was conditioned and introduced into the chamber at the indicated times. The intensity of 510 nm fluorescence induced by sequential excitation of the cells with 408 nm and 488 nm is monitored at 40X magnification with 60-s resolution. Control additions of 10 mM H₂O₂ and 20 mM DTT were made at the end of the exposure period. Plotted is the normalized fluorescence intensity expressed as the ratio of emissions from 405 and 488 nm excitation. These results represent five separate experiments, with values presented as a mean ± SEM for 10 individual cells