Fig. 2

Microvascular endothelial cells have a large and distinct transcriptional response to an ACRE-DEP exposure. RNA-sequencing was performed on the epithelial and endothelial cells after a 6 h and 24 h VEH and ACRE-DEP exposure. A Illustrated representation of the separation of the epithelial and endothelial cells from the ACRE model and removal of the fibroblast layer following an ACRE-DEP exposure. B, C Volcano plots of the differentially expressed genes in the B epithelial cells and C endothelial cells after a 6 h and 24 h ACRE-DEP exposure. The x-axis shows the log₂ fold change of differentially expressed targets in the epithelial and endothelial cells after an ACRE-DEP exposure relative to VEH. The y-axis shows the negative log₁₀ of the two-tailed test P value. Blue dots represent targets with an adjusted p-value of p \(\le\) 0.1. Red dots represent targets with an adjusted p-value of p \(\le\) 0.1 and a log2fold change > 2. D Venn diagrams, sorted by exposure length, of the differentially expressed genes in the epithelial and endothelial cells. E Gene counts of significantly, differentially expressed genes in the epithelial and the endothelial cells. F–H IPA of the top predicted, significantly activated pathways in the F epithelial and endothelial cells after a 6 h (G) and 24 h ACRE-DEP (H) exposure. B–H Represent n = 3 independent experiments. † = Role of hypercytokinemia/hyperchemokinemia in the pathogenesis of influenza