Fig. 4

ACRE-DEP induces alveolar epithelial MAPK signaling and microvascular endothelial NRF2 signaling. A, B Protein expression and densitometry of NRF2, phosphorylated and total ERK1/2, phosphorylated and total p38, and GAPDH or vinculin (loading controls) in A epithelial cells and B endothelial cells over a 2 – 24 h ACRE-DEP exposure. A, B Values represent the mean of n = 3 independent experiments ± SD and immunoblots are representative images from n = 3 independent experiments. Statistically significant differences between vehicle and ACRE-DEP treated cells are indicated by *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001