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Fig. 5 | Particle and Fibre Toxicology

Fig. 5

From: Epithelial MAPK signaling directs endothelial NRF2 signaling and IL-8 secretion in a tri-culture model of the alveolar-microvascular interface following diesel exhaust particulate (DEP) exposure

Fig. 5

Microvascular endothelial antioxidant gene expression is NRF2 dependent. A Illustrated representation of endothelial NRF2 knock down using small interfering RNA (siNRF2). B Protein expression and densitometry of NRF2 stabilization and GAPDH (loading control) in endothelial siNRF2 cells after a 2 h ACRE-DEP exposure. C mRNA expression of the antioxidants HMOX1, NQO1, and GCLM in endothelial siNRF2 cells after a 6 h ACRE-DEP exposure. B, C Values represent the mean of n = 3 independent experiments ± SD and immunoblots are representative images from n = 3 independent experiments. Statistically significant differences between ACRE-DEP and ACRE-DEP + siNRF2 cells are indicated by **p ≤ 0.01 and ****p ≤ 0.0001

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Particle and Fibre Toxicology

ISSN: 1743-8977

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