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Fig. 5 | Particle and Fibre Toxicology

Fig. 5

From: In vitro inflammation and toxicity assessment of pre- and post-incinerated organomodified nanoclays to macrophages using high-throughput screening approaches

Fig. 5

Differentiated THP-1 macrophage cell metabolism, DAMP release, mitochondrial membrane potential, and ROS at 24 h post-exposure in the absence of LPS. A CloisNa and Clois30B exposure both reduced THP-1 cell WST-1 metabolic activity. B CloisNa and Clois30B exposure caused a dose-dependent decrease in live cell counts while C Clois30B exposure caused a dose-dependent increase in cytotoxicity (LDH). D, E Clois30B produced dose-dependent release of DAMPs (IL-1α and ATP) while all other particles had significant minimal or no effect. F Only CloisNa caused a dose-dependent mitochondrial membrane depolarization. G Intracellular reactive oxygen species using CellROX. Points represent mean ± SE. Horizontal dashed bar represents unexposed cell level. * and † indicated significant increase and decrease compared to unexposed cells, respectively (p ≤ 0.05, n = 4)

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Particle and Fibre Toxicology

ISSN: 1743-8977

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