Fig. 7
Representative plots of A LPS and B non-LPS stimulated THP-1 macrophage cell viability metrics significantly correlated to BAL cell differentials at Day 7 post-exposure following pre- and post-incinerated nanoclay particle exposure. Data from all particle types are presented. Most significant correlations were found with THP-1 live cell count in LPS stimulated cells (A) and non-LPS stimulated cells (B; Additional file 3: Table S7). Decreases in live THP-1 macrophages correlated with increased BAL immune cell counts. Comparisons were made at three different in vitro dose equivalent ratios (blue 0.06 and 0.6 µg/cm2; orange 0.2 and 2 µg/cm2; black 0.6 and 6 µg/cm2) versus in vivo aspiration doses (30 and 300 µg /lung) in male C57Bl/6J mice. Particle names and hashed ovals call out noticeable differences between CloisNa, Clois30B, and CS responses