In vitro inflammation and toxicity assessment of pre- and post-incinerated organomodified nanoclays to macrophages using high-throughput screening approaches

Table 1 Key event screening approach in THP-1 macrophages following pre- and post-incinerated organomodified nanoclay exposure

Key event	Stains^a	Fluorescent channels
Live/dead	Propidium Iodide	DAPI, Cy5
Mitochondrial membrane potential	JC-1	DAPI, FITC, Cy3
Reactive oxygen species	CellRox Green	DAPI, FITC
Cell membrane damage^b	LDH	n/a
Cell metabolism^b	WST-1	n/a
Lysosome damage	Magic Red Cathepsin B	DAPI, Cy5
Caspase 1	FAM-FLICA Caspase 1	DAPI, FITC
Caspase 3/7	Cell Event/Propidium Iodide	DAPI, FITC, Cy5
Inflammation response	Multiplex Luminex beads	n/a

^aAll HTS assays, except WST-1 and LDH contained Hoechst 33,342 as a nuclear stain
^bNon-HTS traditional assays

ISSN: 1743-8977