In vitro inflammation and toxicity assessment of pre- and post-incinerated organomodified nanoclays to macrophages using high-throughput screening approaches

Table 3 ED₅₀ comparison (µg/cm²) of pre- and post-incinerated nanoclay exposures to differentiated human THP-1 macrophages in the presence or absence of LPS

Assay	Particle	With LPS	Without LPS
WST-1	CloisNa	> 20*^,a	11.1 ± 3.5
	Clois30B	4.1 ± 0.7	5.4 ± 2.0
	I-CloisNa	> 20*^,a	> 20*^,†,a
	I-Clois30B	> 20*^,a	> 20*^,†,a
	CS	> 20*^{, a}	> 20*^,†,a
Live cell imaging	CloisNa	6.8 ± 2.00	15.2 ± 6.9
	Clois30B	2.4 ± 0.5^b	2.9 ± 0.6^b
	I-CloisNa	11.1 ± 2.5*	> 20*^,a
	I-Clois30B	7.6 ± 1.4*	> 20*^,a
	CS	11.1 ± 03.5*	> 20*^,a

^* and ^†denote those particles with significantly different ED₅₀ values from Clois30B and CloisNa, respectively (p ≤ 0.05, n = 4 independent experiments)
^aEstimated ED₅₀ value was greater than the highest tested dose (20 µg/cm²). Standard error of the mean values were not reported for these estimates
^bClois30B exhibited a non-significant lower ED₅₀ than CloisNa with (p = 0.053) and without (p = 0.062) LPS

ISSN: 1743-8977