Fig. 7

Enhancement of murine hepatic necroptosis and injury after PSNPs injection. BALB/c mice were injected with 60 mg/kg PSNPs alone or together with 50 µM Nec-1. (A) Morphological images of liver tissues. (B) Representative histopathological micrographs of liver sections in mice examined via H&E staining. Scale bar, 50 μm. (C) The severity of hepatic steatosis was determined via Oil red O staining, as indicated by red lipid droplets in hepatocytes. Scale bar, 50 μm. (D) Western blot analysis of necroptosis proteins in liver tissue. (E) Serum ALT, AST and LDH levels. Samples were obtained 24 h post-challenge (n = 6). N.S., no significance. * P < 0.05, ** P < 0.01