Fig. 9

PSNPs induce macrophage-hepatocyte crosstalk cause liver injury. (A) BALB/c mice were preinjected with clodronate liposomes (CLLs) or control liposomes for 3 consecutive days and then challenged with PSNPs for 24 h. The depletion of macrophages was demonstrated by immunofluorescence staining of F4/80 and DAPI in frozen sections of mouse liver. Scale bar, 50 μm (B). Serum ALT and AST levels (C) and liver tissue oxidative damage levels of SOD, -SH, GSH and MDA (D) were detected. n = 3. N.S., no significance. * P < 0.05, ** P < 0.01. 20 nm PSNPs at 50 µg/mL or 60 mg/kg were used in vitro or in vivo respectively