Fig. 7
HO-1 as a crucial player in SiNPs-induced ferroptosis of cardiomyocytes. HO-1 expressions in SiNPs-exposed rat hearts (A) and AC16 cells (B). C Fer-1 inhibited the elevation of HO-1 by SiNPs in AC16 cells. Cells were incubated with siHO-1 and SiNPs (50 μg/mL, 24 h) to verify the role of HO-1 in SiNPs-induced ferroptosis. D Western blot analysis. E Cytosolic Fe2+ level determination using FerroOrange. The fluorescent image (a) was captured, and corresponding fluorescence intensity was analyzed (b). Scale bar, 50 µm. Data were expressed as mean ± SD. *p < 0.05 vs control, #p < 0.05 vs SiNPs