Fig. 8

miR-125b-2-3p targeted HO-1 to mediate ferroptosis triggered by SiNPs. A-B RT-PCR determination and correlation analysis of HO-1 mRNA and miR-125b-2-3p levels upon SiNPs exposure in vivo and in vitro. C Gene sequences of miR-125b-2-3p, and binding sites between miR-125b-2-3p and the 3’-UTR of HO-1. and its connection sites with HO-1. D Relative luciferase activity. miR-125b-2-3p mimics (50 nM) or miR-NC was transfected in AC16 cells, which were then treated with SiNPs (50 μg/mL, 24 h). Western blot analysis E, the cytosolic Fe²⁺ labeling (F-a), and its corresponding analysis (F-b) were performed. Scale bar, 50 µm. Data were expressed as mean ± SD. ^*p < 0.05 vs control, ^#p < 0.05 vs SiNPs