Figure 4From: Amorphous nanosilica induce endocytosis-dependent ROS generation and DNA damage in human keratinocytes Effects of endocytosis and NADPH oxidase inhibitor on generation of ROS induced by silica particle treatment. HaCaT cells were pretreated with cytochalasin D or apocynin for 30 min prior to incubation with 270 mg/ml nSP70 for 3 h. ROS induced by silica particle treatment were expressed as relative fluorescence units, which means that ROS intensity of each silica particle alone and non-treatment is 100 and 0 respectively, in the DCFH assay. Data shown are means ± SD (n = 3). *P < 0.01, **P < 0.05.Back to article page