Figure 6
High surface area particles can interfere with measurement of cytokines in cell-free media containing a known aliquot of recombinant IL-6. A. Addition of 200 μg/mL of nano-SiO2, nano-TiO2, or kaolin to KGM media with the indicated concentration of BSA added. B. Addition of 200 μg/mL of nano-SiO2, nano-TiO2, or kaolin to KGM media with the indicated concentration of bovine serum added. C. The effect of adding increasing amounts of the indicated particles to KGM media without supplemental protein. Measured IL-6 divided by standard, * indicates significantly less than standard prepared in assay diluent, # indicates significantly greater than the IL-6 in the same particle suspension in media without exogenous protein addition, (two-tailed, p < 0.05). N = 3.