- Open Access
A novel method to detect unlabeled inorganic nanoparticles and submicron particles in tissue by sedimentation field-flow fractionation
© Deering et al; licensee BioMed Central Ltd. 2008
Received: 16 April 2008
Accepted: 03 December 2008
Published: 03 December 2008
A novel methodology to detect unlabeled inorganic nanoparticles was experimentally demonstrated using a mixture of nano-sized (70 nm) and submicron (250 nm) silicon dioxide particles added to mammalian tissue. The size and concentration of environmentally relevant inorganic particles in a tissue sample can be determined by a procedure consisting of matrix digestion, particle recovery by centrifugation, size separation by sedimentation field-flow fractionation (SdFFF), and detection by light scattering.
Laboratory nanoparticles that have been labeled by fluorescence, radioactivity, or rare elements have provided important information regarding nanoparticle uptake and translocation, but most nanomaterials that are commercially produced for industrial and consumer applications do not contain a specific label.
Both nitric acid digestion and enzyme digestion were tested with liver and lung tissue as well as with cultured cells. Tissue processing with a mixture of protease enzymes is preferred because it is applicable to a wide range of particle compositions. Samples were visualized via fluorescence microscopy and transmission electron microscopy to validate the SdFFF results. We describe in detail the tissue preparation procedures and discuss method sensitivity compared to reported levels of nanoparticles in vivo.
Tissue digestion and SdFFF complement existing techniques by precisely identifying unlabeled metal oxide nanoparticles and unambiguously distinguishing nanoparticles (diameter<100 nm) from both soluble compounds and from larger particles of the same nominal elemental composition. This is an exciting capability that can facilitate epidemiological and toxicological research on natural and manufactured nanomaterials.
The toxicology of nano-sized (diameter < 100 nm) particles is a topic of current interest because there have been rapid advances in the synthesis of novel nanomaterials for research, consumer, and industrial applications. Recent reviews have discussed nanoparticle health effects [1, 2]. The growing evidence of adverse health effects from exposure to incidentally produced ultrafine particles from combustion and atmospheric processes motivates concern about manufactured nanomaterials. There is epidemiological evidence for cardiovascular effects of ambient ultrafine particulate matter (PM) . Indications that inhaled particles can translocate to the other organs  suggest a link between nanoparticles and neurodegenerative diseases  and other systemic pathologies. Monitoring human exposure to engineered nanoparticles (from air, water, food, consumer products, and soil), determining the rate of particle uptake by humans and food chain organisms, and measuring the resulting nanoparticle concentrations in target organs are major challenges for nanoparticle toxicology studies .
Most nanoparticle uptake and translocation research has quantified nanoparticles in vivo using some type of unique particle label. For example, nanoparticle laboratory studies have included radioactive particles , trace metals such as gold and iridium , and fluorescent particles . However, the population exposures most relevant to health involve the emissions or deliberate release of high-production-volume manufactured nanomaterials and exposures to incidental nanoparticles, such as soot. Combustion emissions and manufactured powders such as fumed silica, ultrafine titanium dioxide (TiO2), and similar industrial materials rarely have a unique and easily detected label.
Examples of current techniques for measuring unlabeled inorganic nanoparticles in animal organs include using electron microscopy to show localization of TiO2 particles to the lung of rats  and using elemental analysis to show the presence of manganese particles in neural tissue . However, measuring changes in the concentration of unlabeled particles in tissue with these techniques is difficult. Extracting quantative information from TEM images is inexact and elemental analysis does not distinguish particles from soluble forms and provides no information on particle size.
A promising method to measure size and concentration of unlabeled nanoparticles is through separation by field-flow fractionation (FFF), which was first developed in the 1960s for separating macromolecules, colloids, and particles [11, 12]. FFF has been used for the measurement of numerous properties of macromolecules and colloidal particles, including particle mass, size, and density. Caldwell et al. reported seminal work applying FFF to detect protein-based particles in eye lens cataracts . FFF has been used to characterize natural aquatic colloids [14–16], and perform size separation of single-walled carbon nanotubes .
FFF is similar to chromatography methods in that materials are separated by transport velocity, but in place of a retention media the separation is carried out in a thin, open channel with bulk flow in the longitudinal direction and a separation field (centrifugal force, electric field, thermal gradients, or cross-flow) in the perpendicular direction. Particles are driven to the wall by the separation field and average particle distance from the wall is determined by the competition between the separation field and the size-dependent diffusion of particles against the concentration gradient. Since the narrow channel has a parabolic flow profile (laminar flow), the particles farthest from the wall are in the highest velocity streamlines and therefore travel the fastest. Sedimentation FFF (SdFFF) uses centrifugal force to generate the separation field. The minimum detectable particle size depends on the particle density and the maximum centrifugal force of the SdFFF instrument [18, 19]. Giddings provides a full derivation of the governing equation and a graph of minimum resolvable diameter versus GΔρ for a typical instrument channel geometry . For example, with the instrument used in this study, silica particles with a density of 2.0–2.65 g/ml and as small as ~22 nm can be separated using the instrument's maximum centrifugal force. For denser particles such as gold, applying the same field can separate particles as small as 10 nm. Multiple detection techniques can be used simultaneously with FFF, including fluorescence, ultra-violet absorption, and light scattering.
In this study tissue lysis and gradient centrifugation, well-established methodologies for the density-dependent separation of subcellular fractions, were adapted to isolate oxide particles from biological samples. Particle isolation was combined with SdFFF to detect and quantify unlabeled inorganic nanoparticles.
Preliminary experiments were conducted to calibrate the instrumentation used in this study and to determine the amount of particles needed for reliable detection. We used dilutions prepared from purchased silicon dioxide (SiO2) standards (Postnova Analytics) with a known particle size, 70 nm, and a starting concentration of 25 mg/ml of particles suspended in aqueous surfactant. With the available light scattering detector, reliable quantification of the standard could be obtained with as few as 7 × 1010 particles per injected sample, which is equivalent to 25 μg of particle mass. Based on these data, our subsequent experimental work used tissue samples containing 1–2 mg of particles. Using particle aliquots greater than 40 times the limit of detection enabled robust quantification in the experiments to develop nanoparticle recovery protocols.
Well established FFF theory  allows the particle size corresponding to a given elution time to be calculated directly from first principles . For SdFFF the calculation is based on measurable physical parameters of the apparatus, the carrier fluid, and the particle density, and involves the equations for settling velocity, particle diffusion rate, and laminar flow profile. Figure 3A shows the theoretical particle sizes (top x-axis labels) corresponding to the measured elution time (bottom x-axis labels) for two different particle densities. These assumed densities, 2.65 and 2.0 g/cm3, correspond to quartz and the density of the 70-nm particles obtained from the vendor datasheet. These assumed densities span a reasonable range for various amorphous and crystalline forms of SiO2. As can be seen from the differences between the two sets of theoretical sizes, the particle size corresponding to a given elution time is not strongly dependent on the assumed density. Thus nanoparticles can be distinguished from micron-sized particles even when the particle composition and density are uncertain. For example, detection of a particle mode within the time range corresponding to SdFFF separation of nano-sized particles for a plausible range of densities would provide useful hypothesis-generating information in a toxicology study of environmental exposures.
Particle recovery for the experiment in figure 3A and 3B can be estimated from the integrated area under the curve for the SdFFF analysis of the tissue sample and the reference sample . Particle recovery in the enzyme digestion processing was 30% for the 250 nm particles and 22% for the 70 nm particles. These recoveries are representative of one set of experimental data.
The goal of this study was to develop a technique that provides detailed information on the size distribution of unlabeled submicron and nano-sized inorganic particles in toxicology samples. Specifically, we wanted to be able show directly by instrumental analysis whether visible particle clusters, such as are shown in Figure 1B, contain nano-sized primary particles. Elemental analysis and radioactive labeled particles provide mass concentration data but not size data. Microscopy-based techniques provide size data only after image analysis of a sufficient sample to get accurate statistics. Manual image analysis is labor intensive and automated image analysis is subject to artifacts from overlapping particles or poor contrast from the background. SdFFF complements the available techniques by providing detailed size distributions from each sample run.
The sample handling methods used this study involved particle dispersion by ultrasound in the presence of a surfactant to demonstrate the presence of a mode corresponding to the primary particle size of the test particle. Characterizing the size distribution of particle aggregates in biological samples is a complex problem that is outside the scope of this pilot study. Particle aggregation is a dynamic process since aggregates are held together by weak surface forces. Aggregate size can be changed by mechanical force as well as by changes in pH, ionic strength, and concentration of surfactants.
A wide range of unlabeled submicron and nano-sized particles of inorganic materials can potentially be detected in tissue samples by the methodology described in this paper. All these proof-of-concept experiments used SiO2 particles, but the technique is directly applicable to a much wider range of particle types, size and shape. The requirements for particle detection by our technique are that the particle be sufficiently dense compared to the carrier fluid, have a different index of refraction from the carrier fluid, and be resistant to the reagents used for tissue digestion and sample cleanup. These requirements are met by essentially all inorganic oxides, pure metal, and elemental carbon-based particle types. Non-spherical particles can also be separated using SdFFF, but mathematical prediction of retention time is more complicated than for spheres. A study using rod-shaped aggregates of latex particles showed that the SdFFF separation time is determined by the maximum dimension of the particle rather than by any average size . Thus, this robust methodology is suitable for use in a wide range of particle toxicology studies that involve correlating biological effects with the concentration of nanoparticles in target organs. The particle size distribution information furnished by SdFFF separation will be uniquely applicable to comparisons of the biological effects of solid particles versus the effects of soluble species, a question that cannot be answered by elemental analysis of ashed or acid-digested samples.
The important question of quantifying human lung burden of combustion-generated nanoparticles provides an example of how the sample preparation and SdFFF separation techniques presented in this paper can be used to complement other methods. Vehicle tailpipe emissions contain submicron particles of carbonaceous material from incomplete combustion and metal oxides from fuel and lubricant additives and engine wear. The carbonaceous primary particulate includes both low-volatility compounds, referred to as organic carbon, and essentially non-volatile large polycyclic molecules, referred to as elemental carbon or black carbon. Grigg et al. conducted a study that used light microscopy to measure black material in lung macrophages of healthy children and correlated this lung burden with lung function and modeled levels of particulate matter . Light microscopy measures the two-dimensional projected area of particle aggregates. This approach is labor intensive, introduces artifacts from the image analysis, and provides no information on the primary particle size distribution or the composition of the opaque material. Saxena et al. recently published a technique for quantitative estimation of diesel and carbon black particles in lung cells based on adapting the thermal-optical-transmittance analytical technique developed for measuring organic and elemental carbon in air pollution samples . This technique provides quantification of the low-volatility and non-volatile carbon by a precise instrumental analysis method, but again provides no information on the primary particle size distribution or on the metal oxide components. In contrast, SdFFF analysis of tissue provides quantitative information on the particle size distribution after dispersal of the recovered particles by sonication in surfactant. Our tissue processing method has the potential of offering high sensitivity since the centrifugation steps allow concentrating the particles from large volume of digested tissue into a small aliquot for analysis. Sequential collection of samples during a SdFFF run is a well established technique . The collected samples, which represent concentrated and size-segregated fractions of the initial particles, can be further analyzed, for example by transmission electron microscopy or elemental analysis. Compared to the other approaches cited above, the tissue digestion and SdFFF approach presented here provides the ability to analyze particle size distribution in large samples, such as a whole lung, and provides information on both carbonaceous and metal oxide particles. Carbonaceous combustion particles have lower density than the silicon dioxide used in this study, but analysis of carbon black by SdFFF has been demonstrated [24, 25]. With additional method development this technique can become a useful tool for studying environmental particle burdens in lungs. Little is known about the background level of naturally formed nanoparticles, and this technique can also be applied to ecosystem studies of nanoparticles in sentinel and food chain organisms.
Considerable future research will be needed to fully realize the potential of our technique for nanoparticle characterization in toxicology studies. Specifically, improvements are needed to reduce particle losses during the enzyme digestion and particle recovery steps of the tissue processing, to make the method sufficiently reproducible, and to permit precise quantification of the nanoparticle burden per weight of original tissue. This paper describes experiments done with relatively high concentrations of the particles because our goal was to demonstrate proof-of-concept. The limit of detection for 70 nm SiO2 particles was 25 μg of particles per SdFFF analysis sample using a light scattering detector. This particle concentration in tissue is within the range reported by in vitro and in vivo nanoparticle toxicity studies. For example, a study of fine and nanoscale quartz particles reported statistically significant responses with an intratracheal instillation dose of 1 mg/kg which equated to an initial burden of about 140 μg of particles per lung . In an inhalation study exposing mice, rats, and hamsters to ultrafine TiO2, the particle burden in the lung was 1.6 mg TiO2/g of tissue immediately post exposure . Thus, the currently demonstrated enzyme digestion and FFF detection methodology is applicable to nanoparticle toxicology studies that use superphysiological doses. However, improvement in the method sensitivity will be needed for use in toxicology studies that use environmentally relevant particle exposures. The tissue processing protocol involves particle recovery by centrifugation and the centrifugation process intrinsically allows a sample to be concentrated. Processing larger initial tissue samples and recovering the particles in a small final volume is a straightforward way to achieve detection of low particle concentrations in tissue.
The capability to detect nanoparticles and to distinguish particle size distribution for unlabeled SiO2 in a sample of mammalian lung tissue has been demonstrated. We have shown that not only can we detect unlabeled SiO2 nanoparticles isolated from rat lung and liver tissue, but more importantly, we distinguished between nano- and submicron-sized particles isolated from the same tissue. The combination of enzyme digestion of tissue with particle sizing by SdFFF is a novel approach that will greatly facilitate measurements of natural and anthropogenic nanoparticles in laboratory toxicology studies, ecological systems, and human populations. This work introduces a new method to characterize the size distribution of unlabeled inorganic particles in tissue which will be useful for studies focused on the neurological and cardiovascular effects of environmental and occupational exposures to an important class of engineered nanomaterials.
We added aliquots of particle suspensions (typically 1–2.5 mg) to homogenized lung or liver tissue. The addition of particles to homogenized tissue demonstrated nanoparticle detection in a complex mixture of biological material without the complications related to in vivo particle distribution and uptake and elimination. Particles were 70 nm diameter SiO2 (Z-PS-SIL-004-0,07, Postnova Analytics Landsberg, Germany) and 250 nm SiO2 (Alfa Aesar, Ward Hill, MA).
Collagenase (150 U/ml) and hyaluronidase (100 U/ml) (Sigma Aldrich) were added to the homogenized tissue to break up the extracellular matrix. The mixture was incubated overnight at 37°C with shaking. The particle-spiked tissue was then sonicated using an Ultrasonic Processor (Cole-Parmer, Vernon Hills, Illinois) for 20 seconds (2 sec bursts). To further break down the proteins we incubated the tissue with 200 μg/ml Proteinase K (Sigma Aldrich) in 0.5% SDS for 2 hrs at 65°C.
The tissue samples were then layered over a saturated sucrose cushion and centrifuged at 21,000 × g for 20 min in a micorcentrifuge (Eppendorf North America, Westbury, NY). The pellets were resuspended in 0.1% FL-70 (Fischer Scientific) and sonicated for 20 sec (2 sec bursts). Phenol was added in a 1:1 (v/v) ratio and incubated with shaking for 5 min followed by another round of centrifugation. The pellet was then resuspended and washed with 70% ethanol and centrifuged. The final pellet was resuspended and sonicated in 0.1% FL-70 with 0.01% sodium azide (Sigma Aldrich).
Analysis of the final samples was done using a Postnova S101 particle fractionator (Postnova Analytics, Salt Lake City, UT). The injected sample volume was 100 μl using 0.1% FL-70 as the carrier fluid at a rate of 2 ml/min. The initial speed of centrifugation was 1800 rpm and the final speed was 200 rpm. SdFFF run time was typically 90 min. Detection was achieved with a light scattering detector (Brookhaven, Holtsville, NY) at a 90° angle (690 nm laser). A companion paper  provides further details of the SdFFF methodology.
Acid digestion was used as an alternative to the enzyme digestion protocol above. The tissue sample was transferred to a glass test tube and an equal volume of 60% nitric acid (Fisher Scientific) was added. The test tube was placed in a beaker of hot (94°C) water for about 1 hour or until the tissue was completely digested. The samples were then centrifuged and the pellet washed with dilute acid and finally resuspended in 0.1% of the aqueous surfactant FL-70.
Treatment of live cell cultures with particles was used as an alternative to adding particles to homogenized tissue, described above. Human aortic endothelial cells (HAEC, Cambrex, Bio Science Walkersville) were cultured in 5% CO2 at 37°C in either a T-25 culture flask (Corning, Corning, NY) or a glass bottom culture dish (MatTek Cultureware, Ashland, MA) in endothelial cell growth medium-2 (EGM-2, Cambrex, Bio Science Walkersville) until 90% confluent. Cells were treated by replacing the media with 4 ml of fresh EGM-2 containing 25 μg/cm2 rhodamine-labeled 70 nm SiO2 (Z-PS-SIL-RFP-0,07 Postnova Analytics Landsberg, Germany) and incubated for 24 hrs. To harvest the cells and the attached or internalized particles for experiments, the culture medium was removed and the cells were washed with phosphate buffer saline (PBS). Following the removal of PBS, 1 ml TrypLE enzyme (Invitrogen) was added and then removed after one minute and incubated for 5 min. The cells were washed from the dish with fresh media and collected by centrifugation at 200 g, resuspended in 500 μl 0.1% FL-70 and sonicated with a probe for 20 seconds (2 sec bursts). The particles and lysed cell contents were then visualized via fluorescence microscopy and then processed via enzyme digestion starting with the Proteinase K step (Figure 4).
Cells were treated with rhodamine-labeled 70 nm SiO2 particles (Z-PS-SIL-RFP-0,07; Postnova Analytics Landsberg, Germany) and fixed in ice cold 100% methanol. The nuclei were stained with DAPI (Molecular Probes). The stained cells were visualized using an Olympus 1 × 50 fluorescent microscope and a Hamamatsu camera. Images were analyzed using ImageJ software.
Transmission electron microscopy (TEM)
Particle samples for TEM were prepared by washing the particles, concentrating by centrifugation, and resuspending in high-purity water. A 5 μL aliquot was placed on a formvar-coated copper grid and allowed to dry overnight. Samples were imaged on a Philips Techni G2 electron microscope at 100 kV.
We would like the thank Postnova Analytics USA for technical assistance and sample analysis, and Nancy Chandler at the Health Sciences Center Electron Microscopy Core for the TEM imaging resources. Support was received from: K25 ES011281, EPA-STAR 8317230 and a University of Utah Synergy Grant for nanoparticle toxicology.
- Balbus J, Maynard A, Colvin V, Castranova V, Daston G, Denison R, Dreher K, Goering P, Goldberg A, Kulinowski K, Monteiro-Riviere N, Oberdörster G, Omenn G, Pinkerton K, Ramos K, Rest K, Sass J, Silbergeld E, Wong B: Meeting report: hazard assessment for nanoparticles–report from an interdisciplinary workshop. Environ Health Perspect 2007, 115: 1654–1659.PubMed CentralView ArticlePubMedGoogle Scholar
- Gwinn M, Vallyathan V: Nanoparticles: health effects-pros and cons. Environ Health Perspect 2006, 114: 1818–1825.PubMed CentralPubMedGoogle Scholar
- Schulz H, Harder V, Ibald-Mulli A, Khandoga A, Koenig W, Krombach F, Radykewicz R, Stampfl A, Thorand B, Peters A: Cardiovascular Effects of Fine and Ultrafine Particles. J Aerosol Med 2005, 18: 1–22. 10.1089/jam.2005.18.1View ArticlePubMedGoogle Scholar
- Kreyling WG, Semmler M, Erbe F, Mayer P, Takenaka S, Schultz H, Oberdörster G, Ziesenis A: Translocation of ultrafine insoluble iridium particles from lung epithelium to extrapulmonary organs is size dependent but very low. Journal of Toxicology and Environmental Health Part A 2002, 65: 1513–1530. 10.1080/00984100290071649View ArticlePubMedGoogle Scholar
- Peters A, Veronesi B, Calderon-Garciduenas L, Gehr P, Chen LC, Geiser M, Reed W, Rothen-Rutishauser B, Schurch S, Schultz H: Translocation and potential neurological effects of fine and ultrafine particles: a critical update. Part Fibre Toxicol 2006., 3:Google Scholar
- Boxall A, Tiede K, Chaudhry Q: Engineered nanoparticles in soils and water: how do they behave and could they pose a risk to human health. Nanomedicine 2007, 2: 919–927. 10.2217/174358188.8.131.529View ArticlePubMedGoogle Scholar
- Sadauskas E, Wallin H, Stoltenberg M, Vogel U, Doering P, Larsen A, Danscher G: Kupffer cells are central in the removal of nanoparticles from the organism. Part Fibre Toxicol 2007, 4: 10. 10.1186/1743-8977-4-10PubMed CentralView ArticlePubMedGoogle Scholar
- Ryman-Rasmussen JP, Riviere JE, Monteiro-Riviere NA: Penetration of intact skin by quantum dots with diverse physicochemical properties. Toxicological Sciences 2006, 91: 159–165. 10.1093/toxsci/kfj122View ArticlePubMedGoogle Scholar
- Geiser M, Rothen-Rutishauser B, Kapp N, Schürch S, Kreyling W, Schulz H, Semmler M, Im Hof V, Heyder J, Gehr P: Ultrafine Particles Cross Cellular Membranes by Nonphagocytic Mechanisms in Lungs and in Cultured Cells. Environmental Health Perspectives 2005, 113: 1555–1560.PubMed CentralView ArticlePubMedGoogle Scholar
- Elder A, Gelein R, Silva V, Feikert T, Opanashuk L, Carter J, Potter R, Maynard A, Ito Y, Finkelstein J, Oberdörster G: Translocation of Inhaled Ultrafine Manganese Oxide Particles to the Central Nervous System. Environmental Health Perspectives 2006, 114: 1172–1178.PubMed CentralView ArticlePubMedGoogle Scholar
- Giddings JC: Unified Separation Science. New York: Wiley-Interscience; 1991.Google Scholar
- Myers MN: Overview of field-flow fractionation. Journal of Microcolumn Separations 1997, 9: 151–162. Publisher Full Text 10.1002/(SICI)1520-667X(1997)9:3<151::AID-MCS3>3.0.CO;2-0View ArticleGoogle Scholar
- Caldwell KD, Compton BJ, Giddings JC, Olson RJ: Sedimentation field-flow fractionation: a method for studying particulates in cataractous lens. Investigative Ophthalmology & Visual Science 1984, 25: 153–159.Google Scholar
- Baalousha M, Lead JR: Characterization of natural aquatic colloids (< 5 nm) by flow-field flow fractionation and atomic force microscopy. Environ Sci Technol 2007, 41: 1111–1117. 10.1021/es061766nView ArticlePubMedGoogle Scholar
- Assemi S, Newcombe G, Hepplewhite C, Beckett R: Characterization of natural organic matter fractions separated by ultrafiltration using flow field-flow fractionation. Water Research 2004, 38: 1467–1476. 10.1016/j.watres.2003.11.031View ArticlePubMedGoogle Scholar
- Lead JR, Wilkinson KJ, Balnois E, Cutak BJ, Larive CK, Assemi S, Beckett R: Diffusion Coefficients and Polydispersities of the Suwannee River Fulvic Acid: Comparison of Fluorescence Correlation Spectroscopy, Pulsed-Field Gradient Nuclear Magnetic Resonance, and Flow Field-Flow Fractionation. Environ Sci Technol 2000, 34: 3508–3513. 10.1021/es991195hView ArticleGoogle Scholar
- Chun J, Fagan J, Hobbie E, Bauer B: Size separation of single-wall carbon nanotubes by flow-field flow fractionation. Analytical Chemistry 2008, 80: 2514–2513. 10.1021/ac7023624View ArticlePubMedGoogle Scholar
- Schimph ME, Caldwell KD, Giddings JC, Eds: Field-Flow Fractionation Handbook. New York: John Wiley & Sons, Inc; 2000.Google Scholar
- Takjiki S, Assemi S, Deering CE, Veranth JM, Miller JD: Detection, separation, and quantification of unlabeled silica nanoparticles in biological media using sedimentation field-flow fraction. J Nanoparticle Research accepted for publication 2008.Google Scholar
- Giddings JC, Ratanathanawongs SK, Moon MH: Field-Flow Fractionation: A Versatile Technology for Particle Characterization in the Size Range 10-3to 102micrometers. Kona: Powder and Particle 1991, 9: 200–217.View ArticleGoogle Scholar
- Blau P, Zollars RL: Sedimentation Field-Flow Fractionation of Nonspherical Particles. Journal of Colloid and Interface Science 1996, 183: 476–483. 10.1006/jcis.1996.0570View ArticlePubMedGoogle Scholar
- Grigg J, Kulkarni N, Pierse N, Rushton L, O'Callaghan C, Rutman A: Black-Pigmented Material in Airway Macrophages from Healthy Children: Association with Lung Function and Modeled PM10. Res Rep Health Eff Inst 2008, (134):1–23.Google Scholar
- Saxena RK, Gilmour MI, Hays MD: Isolation and quantitative estimation of diesel exhaust and carbon black particles ingested by lung epithelial cells and alveolar macrophages in vitro. Bio Techniques 2008, 44: 799–805.Google Scholar
- Kim Ws, Kim SH, Lee DW, Lee S, Lim CS, Ryu JH: Size Analysis of Automobile Soot Particles Using Field-Flow Fractionation. Environ Sci Technol 2001, 35: 1005–1012. 10.1021/es001329nView ArticlePubMedGoogle Scholar
- Park YH, Kim WS, Lee DW: Size analysis of industrial carbon blacks by sedimentation and flow field-flow fractionation. Anal Bioanal Chem 2003, 375(4):489–495.PubMedGoogle Scholar
- Warheit DB, Webb TR, Colvin VL, Reed KL, Sayes CM: Pulmonary bioassay studies with nanoscale and fine-quartz particles in rats: Toxicity is not dependent on particle size but on surface characteristics. Toxicological Sciences 2007, 95: 270–280. 10.1093/toxsci/kfl128View ArticlePubMedGoogle Scholar
- Bermudez E, Mangum JB, Wong BA, Asgharian B, Hext PM, Warheit DB, Everitt JI: Pulmonary responses of mice, rats, and hamsters to subchronic inhalation of ultrafine titanium dioxide particles. Toxicological Sciences 2004, 77: 347–357. 10.1093/toxsci/kfh019View ArticlePubMedGoogle Scholar
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