Diesel exhaust modulates ozone-induced lung function decrements in healthy human volunteers
© Madden et al.; licensee BioMed Central Ltd. 2014
Received: 14 January 2014
Accepted: 5 August 2014
Published: 2 September 2014
The potential effects of combinations of dilute whole diesel exhaust (DE) and ozone (O3), each a common component of ambient airborne pollutant mixtures, on lung function were examined. Healthy young human volunteers were exposed for 2 hr to pollutants while exercising (~50 L/min) intermittently on two consecutive days. Day 1 exposures were either to filtered air, DE (300 μg/m3), O3 (0.300 ppm), or the combination of both pollutants. On Day 2 all exposures were to O3 (0.300 ppm), and Day 3 served as a followup observation day. Lung function was assessed by spirometry just prior to, immediately after, and up to 4 hr post-exposure on each exposure day. Functional pulmonary responses to the pollutants were also characterized based on stratification by glutathione S-transferase mu 1 (GSTM1) genotype. On Day 1, exposure to air or DE did not change FEV1 or FVC in the subject population (n = 15). The co-exposure to O3 and DE decreased FEV1 (17.6%) to a greater extent than O3 alone (9.9%). To test for synergistic exposure effects, i.e., in a greater than additive fashion, FEV1 changes post individual O3 and DE exposures were summed together and compared to the combined DE and O3 exposure; the p value was 0.057. On Day 2, subjects who received DE exposure on Day 1 had a larger FEV1 decrement (14.7%) immediately after the O3 exposure than the individuals' matched response following a Day 1 air exposure (10.9%). GSTM1 genotype did not affect the magnitude of lung function changes in a significant fashion. These data suggest that altered respiratory responses to the combination of O3 and DE exposure can be observed showing a greater than additive manner. In addition, O3-induced lung function decrements are greater with a prior exposure to DE compared to a prior exposure to filtered air. Based on the joint occurrence of these pollutants in the ambient environment, the potential exists for interactions in more than an additive fashion affecting lung physiological processes.
Numerous epidemiological studies have demonstrated an association between short-term exposure to ambient airborne particulate matter (PM) and adverse cardiopulmonary effects including premature mortality, increased hospitalizations for lung problems including infections, exacerbation of asthma symptoms, chronic bronchitis, and hospitalization for clinical cardiac events including arrhythmias, myocardial infarctions, and congestive heart failure ,. The health effects are more strongly associated with PM that is smaller than 2.5 μm, i.e. PM2.5, which typically is derived from human based activities such as vehicular emissions. PM2.5 is a complex mixture of organic and inorganic compounds absorbed onto carbonaceous material with the composition varying across space and time. In this complex mixture of ambient air substances, the ubiquitous pollutants ozone (O3) and diesel exhaust (DE) can be can be major and important components. DE can have "hotspots" such as bus terminals and major streets . Levels of DE PM2.5 reached transient concentrations of several hundred μg/m3 during drive-by studies . O3 levels have generally been decreasing in the US, but can reach over 0.1 ppm on a regular basis.
Ozone is one of the best studied gas phase pollutants in terms of lung biological effects. With dozens of controlled human exposures, biological responses have been well documented particularly for induction of decrements of lung function and inflammation typically expressed as decreased FEV1 and FVC  and lung neutrophilia ,. in a concentration-response manner (typically 0.08-0.4 ppm). Repeated exposure studies with healthy young adults inhaling 0.4 ppm O3 for 2 hrs have shown a decrement in lung function after the first exposure and an even greater decrement after a second day exposure, but then attenuated lung function decrements for the next 3 exposure days ,. Some reports have linked certain genetic polymorphisms to possible sensitivities to O3 exposure. For example, individuals exercising on a bike outdoors for 2 hr with ambient O3 levels > 0.08 ppm had greater lung function decrements as a group when they possessed a glutathione-S-transferase mu 1 (GSTM1) null genotype coupled with wild type NQO1 compared to other combinations of those 2 gene polymorphisms . BMI has also been proportionally associated with O3-induced lung function decrements .
Lung function decrements are typically not induced by exposure to DE or PM alone in controlled study designs . Controlled ambient PM exposure studies in humans have been used to examine biological responses as surrogates for understanding the health effects and mechanisms involved in the responses. A dose-dependent increase in total number of cells, neutrophils, and monocytes was observed in the lung lavage fluid in contrast to lung function. For instance, healthy human volunteers exposed to 300μg/m3 DE for one hour with intermittent exercise resulted in marked systemic and pulmonary inflammatory responses ,.
There is interest in examining whether ambient pollutant gases can modify the biological effect induced by PM . A handful of controlled exposure studies have shown an interactive effect of PM and O3 exposure especially with blood and cardiac endpoints. For instance blood pressure increases have been noted with co-exposure of healthy and also asthmatic subjects to O3 and concentrated ambient particles (CAPS) . Increased vasoconstriction in humans occurred upon exposure to O3 with CAPs . Rodent models of multipollutant exposures have also been utilized to examine biological responses. In an allergic mouse model, both DE and O3 were needed to increase lung resistance, which was not observed with individual pollutant exposures . Exposures of rats to resuspended DE particles and O3 induced changes in some aortic mRNA  suggesting changes induced by pollutant components traversing the lung.
There is the possibility that exposure to one pollutant may sensitize an individual in a manner that a biological response from an exposure to a second pollutant may be enhanced. Sequential O3 and DE exposures in healthy young adult participants in a controlled exposure setting has demonstrated that an initial exposure to 300μg/m3 DE for 1 hr followed 5 hr later by a 2 hr exposure to 0.2 ppm O3 induced more lung inflammation (e.g. neutrophils) than DE alone with no O3 exposure . The same research group, using a similar exposure regimen, showed that a prior exposure to DE increased O3-induced lung inflammation relative to O3 exposure alone . However, these studies did not report on lung function changes. Combinations of exposure to sulfuric acid aerosols and O3 have demonstrated slightly additive lung function changes in asthmatic subjects  but not in healthy individuals . This study primarily examined whether combinations of exposures to O3 and DE can induce additive or synergistic effects related to lung function changes in healthy, normal adults, whether exposure to DE alters the response to a subsequent exposure to O3 approximately 20 hrs later, and whether GSTM1 genotype status has an effect on the lung function responses.
Materials and Methods
Subject characteristics of participants in the study
27 ± 4
27.63 ± 3.95
All exposures were carried out at the EPA Human Studies Facility in Chapel Hill, NC. The targeted exposure atmosphere was approximately 40 ± 10% RH and 22 ± 2°C. Outdoor air was passed through an air purification system consisting bed of Purifil (potassium permangate on alumina), a bed of Purikol (activated charcoal), dehumidified, and passed over an unheated bed of Hopcalite and then through a HEPA filter to ensure no detectable presence of pollutant gases (O3, NO, NO2, SO2, CO, and hydrocarbons with the exception of methane) in the FA. The air was then heated, humidified, and passed through a second HEPA filter before O3 and DE were added. The concentrations of the pollutant gases and PM were monitored continuously. The DE was generated from a Cummins diesel engine/generator QSB7 series (model DSGAA) running at 50 percent load that was located outside the Facility, and subsequently introduced into the exposure chamber after dilutions with humidified FA to give a chamber concentration target of approximately 300μg/m3. The DE particle concentration was controlled by modulating the flow of medical grade compressed air into a Vortec Transvector Air Flow Amplifier (Model 901XSS; Cincinati OH) with real time TEOM monitoring of PM mass concentration. Diesel fuel used for the study was a commercially obtained ultra low sulfur (<15 ppm) fuel. Particle mass, numbers, and size characteristics were measured in real time by a DataRAM™, a Tapered Element Oscillating Microbalance (TEOM®), and a Condensation Particle Counter (CPC). The CPC TSI 3022A model (detection size limit 7 nm) was used for the first 11 subjects’ exposures, and a TSI 3775 model (detection size limit 4 nm) was used for the last 4 subjects’ exposures for detection of particle number concentration. A Scanning Mobility Particle Sizer (SMPS™) (Shorewood MN) as also used to measure particulate characteristics during subject exposures. The SMPS data were used to determine particle size distribution as a number median diameter in all size bins. The number median diameter is the particle size (μm) bin where the median of the total count number occurs. Particle size distribution was determined at regular intervals. A Versatile Air Pollution Sampler (VAPS) (URG, Chapel Hill NC) configured with one quartz and two teflon filters with flow controlled by a mass flow controller sampled inlet air to the chamber. After exposure and filter weight determination, filter samples were analyzed for chemical composition of particles. Elemental (EC) and Organic (OC) Carbon content were determined using a thermo-optical method based on sequential pyrolytic vaporization and detection by transmittance using a carbon analyzer (Model 107A, Sunset Laboratory Inc., Hillsborough, NC) . A bacterial endotoxin characterization test on particles was conducted by Cape Cod Inc. (East Falmouth, Massachusetts) using a Limulus polyphemus gel clot method to test for endotoxin contamination following their standard operating procedures.
Ozone was generated by passing USP grade oxygen through a silent arc O3 generator (Innovatee Geratetechnmik GmbH, Rheinbach Germany) (Model CMG 10−5 for the DE chamber (Day 1) and Model 500 for the O3 chamber on Day 2). For Day 1 exposures O3 entered the filter air stream before DE. Concentrations were controlled by a computer feedback to the O3 generator with a 0-5 V signal allowing internal modulation control of the O3 generator. Both DE and O3 were delivered into the exposure chamber within 8 sec to minimize DE loss. Increased production of O3 was needed when DE was present as NO reacted with O3.
The primary hypothesis of this study was to examine whether combinations of exposures to O3 and DE induce synergistic effects on lung function and whether exposures to DE sensitize individuals for a larger response to O3 than does the exposure by air. To evaluate changes between pollutant and filtered air exposures we used a full two-factor (arm b plus arm c (O3 plus DE), and arm d (O3 + DE)) mixed effects model with a subject-specific random intercept to account for repeated measures. To examine the first hypothesis with respect to synergism of effects, changes in lung function were examined following Day 1 exposures. To examine whether exposures to DE sensitize individuals for a larger response to O3, lung function was examined following O3 exposure on Day 2. In both analyses changes in lung function were contrasted to null hypothesis of no change relative to pre exposure baseline and relative to air exposure as control. Prior to the analysis, lung function endpoints FEV1 and FEV were normalized and expressed as percent change from the pre-exposure values (Post/Pre-1). All 15 subject subjects completed four exposures on Day 1. Following O3 exposure on day 1 some subjects responded with large drop in lung function and were not asked to undergo a Day 2 O3 exposure. On day 2, 15 subjects completed O3 exposures following air and DE (arms a and b, respectively), 14 of these completed O3 exposure following O3 on Day 1 (arm c), and 11 completed Day 2 O3 exposure following of the combined O3 & DE exposure (arm d). We used all available data in the analysis and did not account for possibly non-random missing values in subjects who responded strongly to O3 or O3 & DE on day 1. Estimated mean changes in lung function within each exposed group at each time point are expressed as mean change ± standard error, and a p-value where applicable. All statistical analyses were performed using R statistical software, with nlme and gmodes packages to estimate the effect (version 3.0.1; R Developement Core Team; http://www.r-project.org/). A p value of less than 0.05 was considered statistically significant.
Mean Day 1 pollutant physicochemical parameters during the four exposure scenarios
DE + O3
PM (μg/m 3 ) a
1.0 ± 1.5
1.0 ± 1.1
297. 1 ± 20.8
294.3 ± 20.1
PM # (x10 3 /cc)
731 ± 90b (11)
734 ± 50b (11)
977 ± 55c (4)
903 ± 306c (4)
Median PM size
0.200 ± 0.007
0.200 ± 0.007
Number (μm) d
0.064 ± 0.006
0.066 ± 0.003
O 3 ppm
0.00 ± 0.00
0.30 ± 0.00
0.01 ± 0.00
0.30 ± 0.00
0.09 ± 0.10
0.09 ± 0.10
2.58 ± 0.25
2.51 ± 0.32
0.00 ± 0.01
0.01 ± 0.03
1.58 ± 0.26
0.03 ± 0.04
0.00 ± 0.00
0.00 ± 0.00
0.16 ± 0.05
1.72 ± 0.22
2.05 ± 0.10
1.98 ± 0.10
2.35 ± 0.14
2.24 ± 0.19
Lung function after exposures to pollutants on Day 1
Among the Day 1 exposures statistically significant (p < 0.01) FVC decrements were observed in O3-exposed subjects (8.7 ± 2.3%) and DE + O3 exposed subjects (11.8 ± 3.0%) immediately post-exposure compared to FA exposures (Figure 2B). The FVC decrements immediately post O3 compared to post DE + O3 exposure were not statistically different (p = 0.10), nor was greater than additive effects observed when FVC changes in arm d were compared to the sum of FVC changes post O3 and DE. Exposure to either air or DE alone did not alter FVC from pre-exposure values. FVC values returned to pre-exposure levels by 4 hrs post-exposure for all exposure groups with no difference in the recovery rate (data not shown).
Lung function after exposure to O3 on Day 2
Some subjects were not sequentially exposed to Day 2 O3 either due to their request or due to their large decrement in FEV1 immediately after either O3 (n = 1) or DE + O3 (n = 4) on Day 1 because a second sequential O3 exposure within 24 hr is expected to typically induce an even larger FEV1 decrement than the first day exposure in most subjects. Consequently, for those five subjects Day 2 procedures were follow-up measurements and observations, similar to Day 3 measurements for the other 10 subjects. Subjects in this study had a greater O3-induced lung function decrements if they received two consecutive days of O3 exposure (arm c); consecutive O3 exposures induced a statistically significant greater decrement in FEV1 (18.2 ± 4.5%) than the decrement associated immediately after the first day exposure, (i.e., 10.3 ± 3.3%,) (p < 0.05) (Figure 4A) or compared to the Day 1 air exposure, then O3 on Day 2 (i.e., 10.9 ± 2.6%) . The FVC decrement (7.8 ± 1.8%) on Day 2 following air (arm a) (Figure 4B) was similar to the Day 1 FVC decrement (8.7%) after O3 (Figure 2B). For subjects exposed to DE + O3 (arm d) the FEV1 decrement immediately after exposure to O3 on Day 2 was 13.8 ± 2.2% , which was similar to the decrement in these subjects the previous day immediately after exposure (i.e., 17.6 ± 4.6%). The FVC decrement on Day 2 (Figure 4B) was similar to the Day 1 FVC decrement as well (Figure 2B). Both FEV1 and FVC values returned to pre-exposure values by 4 hr post exposure (data not shown).
When FEV1 responses to the Day 2 O3 exposure were segregated by GSTM1 genotype, it was observed that those subjects with the null genotype had a smaller decrement (3.8 ± 3.8%) than positive genotypes (14.9 ± 3.6%) after the FA exposure day; however this difference was not significant. This is a consistent effect with the observed Day 1 responses to O3 exposure between the two groups. No other Day 2 FEV1 differences were observed between genotypes for the other 3 exposure regimens from Day 1.
This study examined alterations in lung function after co-exposures of DE and O3, as well as sequential exposures to DE and O3 separated by one day. For the former exposure regimen, DE alone did not induce lung function as measured by FEV1 and FVC decrements, but O3 did induce decrements after 2 hr reaching statistical significance for FEV1. Exposure of the subjects to the combination of the two pollutants (arm d) induced a greater FEV1 decrement than either pollutant alone. If the pollutant-induced FEV1 changes are added together, i.e., the sum of the changes in FEV1 post O3 and post DE exposures, and compared to the combined exposure (arm d), the p value of 0.057 is obtained and is close to statistical significance (Figure 2A). A possible explanation for the synergistic effect induced by DE + O3 co-exposure may be that the lung function change is related to the chemical composition in the atmosphere containing O3 and DE being different from DE or O3 alone. DE-derived particle size did not change with the presence of O3, and chamber temperature and humidity were kept similar between the two exposure regimens, suggesting that particle deposition pattern in the lung was not likely altered due to a size change. However, the possibility that other PM physicochemically related characteristics were altered, such as a change in charge or hydrophobicity that could alter the deposition pattern cannot be excluded. For all exposures regimens, lung function returned to pre-exposure values by 4 hrs after the end of the exposure, similar to the well characterized and transient nature of O3-induced lung function changes.
With respect to component changes in the gas phase relating to the greater than additive effect of O3 and DE on FEV1 decrement, the NO2 level increased while the NO level decreased. However, NO2 alone generally does not induce lung function changes even up to 4 ppm in healthy individuals  though we cannot totally exclude the possibility that it contributed to changes in lung function seen during co-exposure to DE and O3. O3 concentrations were kept at ~ 0.300 ppm with or without DE present, so the only other components with potentially altered concentrations were derived from DE. If lung function responses to O3 exposure are mediated at least in part via C-fiber activation  then chemical components affecting C-fibers activity directly (but below a threshold for discharge), or indirectly by, e.g., lowering the fiber threshold for electrical discharge, might be expected to induce the synergistic change in FEV1 seen upon concurrent O3 and DE exposure. Some carbonyls, (e.g., acrolein) and carboxylic acids (e.g., acetic acid) have been implicated in affecting pulmonary C-fiber activity –. There are several aldehydes identified in DE including acrolein, and there is increased formation of carbonyls and carboxylic acids observed upon O3 reacting with DE particles surface components . There is evidence for a possible increased carbonyls and/or carboxylic acids formation and induction of C-fiber sensitization in the DE and O3 atmosphere, but other classes of compounds cannot be eliminate either.
Subjects in this study receiving DE exposure the day before the Day 2 O3 exposure had greater FEV1 decrements than when they had a Day 1 clean air exposure (Figure 5). It is uncertain whether only the DE gas phase or particle phase, or both phases, are needed to induced the augmented response to a subsequent O3 exposure. Most previous studies examining sequential exposure effects of air pollutants on O3-induced lung effects changes were typically performed within 6 hr of the initial exposure ,,. A report demonstrated that prior exposure for 2 hr to 0.6 ppm NO2 with intermittent exercise (~40 L/min) did not significantly alter lung function in the young, healthy subjects; however compared to air as the initial exposure, the pre-exposure to NO2 induced a 1.8% greater FEV1 decrement upon a 2 hr exposure to 0.300 ppm O3 3 hr post NO2 exposure . Another study reported that a same day follow up to approximately 0.200 ppm O3 added to the lung inflammatory response (i.e., increased neutrophilia and myeloperoxidase) induced by an initial 300 ug/m3 DE exposure compared to an initial air exposure; but lung function was not examined in that study . A possible mechanism involved with the augmented FEV1 change to O3 on Day 2 in this study may involve, at least in part, altered production of prostaglandins E2 (PGE2) and F2α (PGF2α) which have been associated with O3-induced lung function decrements . Both prostaglandins can alter lung C-fiber activity directly inducing discharge and/or altering the threshold for discharge to an agonist  The possible altered AA metabolism in response to sequential DE and O3 exposures (i.e., increased AA release, decreased re-uptake, and COX-2 protein for increased conversion of AA to PGE2 and F2α), based upon in vivo and in vitro findings –, may explain, at least in part, the observed greater FEV1 decrement. It should be noted that an adequate duration of time is needed for COX-2 protein to be upregulated . Additionally the literature suggests that the DE particle phase is involved in this possible biochemical mechanism (i.e., increased COX-2), but does not necessarily negate the importance of DE gas phase components.
Factors that affect an individual’s sensitivity to O3-induced lung function decrements are not clearly understood. Increased BMI has been linked to a greater O3-induced lung function decrement though predominantly driven by females with BMI > 25 . In our study no statistically significant changes were observed in the whole population, and with only four female study participants the analyses was likely underpowered. Certain genotypes, including GST isozymes, have been examined as possible biomarkers of sensitivity. Specifically for this study, GSTM1 genotype status was examined as the null genotype was not associated with a greater FEV1 decrease upon exposure of young, normal, healthy adults to a relatively low 0.06 ppm O3 concentration for 6.6 hr with exercise (24), though lung neutrophilia was associated with the allele deletion. In our study, the GSTM1- subjects had an approximately 10% smaller decrement to 0.300 ppm O3 exposure than GSTM1+ subjects in arm c Day 1 and arm a Day 2, though the difference did not reach statistical significance. However, no increased resistance to development of an FEV1 decrease was observed in the GSTM1- group upon the co-exposure to DE and O3, possibly due to a similar sensitivity to DE components or DE components that were influenced by O3.
The modulation of O3-induced lung function changes by DE exposure raise the possibility that such interactions may occur from exposures to ambient levels of these pollutants. Additionally the components in DE that altered the magnitude of the O3-induced FEV1 changes may be also present in our environment and potentially derived from natural or anthropomorphic sources and not exclusively from DE. It is unclear at what ambient concentrations DE may affect responsiveness to O3 exposure, and also the threshold of O3 level that induces lung function decrements; recent literature suggest it may be as low as the 0.06-0.08 ppm range ,. Additionally populations sensitive to the DE-induced lung function effects have not been fully identified, and remains an ongoing issue.
We gratefully acknowledge the help and support of Heidi Hiers of the US EPA Medical Staff, Ana Rappold (US EPA) for biostatistical support, the Westat recruitment staff, and the TRC engineering staff in completing the study.
This report has been reviewed by the National Health and Environmental Effects Research Laboratory, United States Environmental Protection Agency and approved for publication. Approval does not signify that the contents necessarily reflect the views and policies of the Agency nor does mention of trade names or commercial products constitute endorsement or recommendation for use. None of the authors has any actual or potential competing financial interests.
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