Fig. 4
Effect of NPs and NP-conditioned media on COC expansion. After incubation of NPs for 16 h in culture medium, the resulting conditioned medium, deprived of NPs through centrifugation, was used to culture COCs (upper panel). In parallel, COCs cultures were performed in the presence of the indicated NPs (lower panel). In all samples, the extent of COC expansion was assessed after 16 h incubation, and expressed as expansion index (Ei)