Evolutionary age of repetitive element subfamilies and sensitivity of DNA methylation to airborne pollutants
© Byun et al.; licensee BioMed Central Ltd. 2013
Received: 3 January 2013
Accepted: 5 July 2013
Published: 15 July 2013
Repetitive elements take up >40% of the human genome and can change distribution through transposition, thus generating subfamilies. Repetitive element DNA methylation has associated with several diseases and environmental exposures, including exposure to airborne pollutants. No systematic analysis has yet been conducted to examine the effects of exposures across different repetitive element subfamilies. The purpose of the study is to evaluate sensitivity of DNA methylation in differentially‒evolved LINE, Alu, and HERV subfamilies to different types of airborne pollutants.
We sampled a total of 120 male participants from three studies (20 high-, 20 low-exposure in each study) of steel workers exposed to metal-rich particulate matter (measured as PM10) (Study 1); gas-station attendants exposed to air benzene (Study 2); and truck drivers exposed to traffic-derived elemental carbon (Study 3). We measured methylation by bisulfite-PCR-pyrosequencing in 10 differentially‒evolved repetitive element subfamilies.
High-exposure groups exhibited subfamily-specific methylation differences compared to low-exposure groups: L1PA2 showed lower DNA methylation in steel workers (P=0.04) and gas station attendants (P=0.03); L1Ta showed lower DNA methylation in steel workers (P=0.02); AluYb8 showed higher DNA methylation in truck drivers (P=0.05). Within each study, dose–response analyses showed subfamily-specific correlations of methylation with exposure levels. Interaction models showed that the effects of the exposures on DNA methylation were dependent on the subfamily evolutionary age, with stronger effects on older LINEs from PM10 (p‒interaction=0.003) and benzene (p‒interaction=0.04), and on younger Alus from PM10 (p-interaction=0.02).
The evolutionary age of repetitive element subfamilies determines differential susceptibility of DNA methylation to airborne pollutants.
KeywordsEnvironment Exposures DNA methylation Repetitive elements Subfamily
Observed and expected CpG content
Human endogenous retrovirus
- LINE-1 or L1:
Long interspersed nuclear elements
Polymerase chain reaction
Short interspersed nuclear element family.
Approximately half of the human genome is made of repetitive elements . Repetitive elements, particularly L1s, are DNA sequences that only encode for proteins instrumental to their replication and insertion into new locations within the genome. Also often referred to as “junk DNA”  or “jumping genes” , repetitive elements have been active in mammalian genomes for over 100 million years and are therefore a source of evolution and genome structure organization. Repetitive elements are believed to have reshaped the human genome through continuous jumping to remote genomic locations and through the activation of alternative transcription of nearby genes [4–7]. De novo retrotransposition insertions occurring in the germ line are inherited through generations. However, many newly inserted repetitive elements have been observed in somatic tissues and, albeit non heritable, have been linked with human disease and cancers [8, 9].
Among repetitive elements, the best studied families are the long interspersed nuclear elements (LINE-1 or L1), short interspersed nuclear element family (SINEs), and human endogenous retrovirus (HERV) family. Alu and long-terminal repeats (LTRs) are the most abundant representatives of SINEs and HERVs, respectively. All these families are classified as retroelements because of their ability to retrotranspose, i.e., copy themselves into an RNA intermediate and insert back into the genome as a new cDNA copy . Among repetitive elements, only LINE-1 and Alu have been unequivocally shown to be still active and retrotransposition-competent in the current human genome . Since the mobilization of repetitive elements has been linked to genomic instability and consequent genetic disorders, mechanisms such as DNA methylation are believed to have developed in cells to control the proliferation of retrotransposons. CpG-dinucleotide DNA methylation in repetitive elements has been proposed to contribute to repress retrotransposition activity as part of a self-defense system of the host genome [12, 13]. On the other hand, decreased DNA methylation in repetitive elements has been linked with increased transcription and higher rates of retrotransposon activity in vitro[14, 15]. In human studies, differences in DNA methylation of LINE-1, Alu, and HERV have been consistently demonstrated in response to stress  and infections , as well as in autoimmune diseases , cardiovascular diseases  and cancers [20, 21].
Through their “copy and paste” retrotransposition activity, each repetitive element family has gradually accumulated new base substitutions, insertions or deletions referred to as “diagnostic mutations” , which allow for distinguishing various subfamilies within each repetitive element family. Through the analysis of these mutations and the divergence of the repetitive element consensus sequences from the original ancestral sequence, it is possible to estimate an “evolutionary age” for each subfamily [1, 23]. The mutation rate depends on the CpG density; since CpG di-nucleotides are more sensitive than non-CpG sites, they tend to be eliminated through evolution by substitution to either TpG or CpA dinucleotides. These substitutions are frequent events, as the mutation rate of CpG sites is 9.2 fold faster than non-CpG changes [24, 25]. In the mammalian genome, CpG dinucleotides are found to be highly represented in repetitive elements . Through many cycles of substitutions, old subfamilies remain less rich in CpGs and show weakened or no retrotransposon activity, whereas young subfamilies are richer in CpGs and still transcriptionally active in the human genome [25, 27].
Recent investigations have repeatedly linked DNA methylation of repetitive elements with environmental exposures. In human studies, methylation of repetitive elements in blood DNA has been correlated with a number of exposures that generate oxidative stress and inflammation [28, 29], including airborne pollutants [21, 30–32], metals [33–35], and persistent organopollutants [36, 37]. Several investigators have proposed that these effects may contribute to generate – or at least reflect – the altered retrotransposon methylation states related with aging, autoimmune diseases (e.g., multiple sclerosis, lupus), cardiovascular disease, or cancer development and progression . DNA methylation of repetitive elements has been also widely proposed as an indicator of global genomic methylation level . However, current investigations have only focused on the analysis of a single common sequence for each family, which is not expected to comprehensively reflect DNA methylation patterns within specific subfamilies. Recent reports have shown that methylation of an individual common sequence is not correlated with the global content of methylation in normal tissues . A recent observational study showed that CpG content is a primary predictor of changes over time in DNA methylation at individual CpG sites . Because repetitive element CpG content and DNA methylation levels vary dramatically across subfamilies with different evolutionary age, repetitive element subfamilies may also have differential sensitivity to environmental exposures. However, no systematic analysis has yet been conducted to examine the effects of environmental exposures across different subfamilies of repetitive elements.
Characteristics and exposure levels of the study participants
Highly-exposed steel workers (n=20)
Low-exposed controls (n=20)
Study 1 a Exposure to metal-rich particulate matter (PM)
Mean ± SD
203.7 ± 22.9
100.9 ± 28.9
[152.2 ; 227.9]
[73.7 ; 150.0]
Age [Years], mean ± SD
42.4 ± 7.9
37.8 ± 3.0
Ex/current smokers, n (%)
Study 2 b Exposure to air benzene
Gas station attendants (n=20)
Low-exposed controls (n=20)
Air benzene [μg/m3]
Mean ± SD
78.6 ± 42.5
7.0 ± 5.5
[31.2 ; 180.1]
[4.2 ; 23.0]
Age [Years], mean ± SD
39.9 ± 11.2
39.7 ± 10.4
Ex/current smokers, n (%)
Study 3 c Exposure to traffic-derived elemental carbon
Truck drivers (n=20)
Low-exposed controls (n=20)
Elemental carbon [μg/m3]
Mean ± SD
21.3 ± 4.7
13.4 ± 2.1
[16.6 ; 35.6]
[7.8 ; 16.1]
Age [Years], mean ± SD
35.2 ± 5.1
33.4 ± 6.0
Ex/current smokers, n (%)
Repetitive element evolutionary age, CpG density and methylation levels
Characteristics of the repetitive element subfamilies examined in the present study
Evolutionary age (Mya)a
CpG density (CpGo/e)b
Mean methylation (%)c
DNA methylation of repetitive element subfamilies by exposure group
Dose–response relationship in the correlation of DNA methylation in repetitive element subfamilies with exposure levels
Dose–response relationship between levels of personal air pollutants exposure and DNA methylation in the participants
Study 1 associations with metal-rich particulate matter (PM10)
Study 2 associations with air benzene
Study 3 associations with traffic-derived elemental carbon
Evolutionary age of subfamilies and sensitivity of DNA methylation to airborne pollutants
In Study 1, the negative effect of PM10 exposure on LINE-1 methylation was stronger with increasing evolutionary age (τ for interaction=-0.6; P=0.003) (Figure 3A). Also in Study 1, the interaction model showed a positive significant interaction between PM10 exposure and evolutionary age in predicting Alu family methylation (τ=0.2 and P=0.017) (Figure 3B). However, as shown in the slopes in Figure 3, the differences of the effects of PM10 in Study 1 across different evolutionary ages were more pronounced for LINE-1 methylation (Figure 3A) compared to Alu methylation (Figure 3B). In Study 2, the negative effect of airborne benzene in LINE-1 DNA methylation was also progressively stronger with increasing evolutionary age (τ for interaction=-0.2; P=0.045) (Figure 3C). No interactions were found between evolutionary ages and exposure levels in determining HERV methylation (see Additional file 1: Table S2).
DNA methylation of repetitive elements has been extensively studied in relation to environmental exposures and human disease. Nonetheless, most if not all of the previous studies have investigated one single sequence in one or at most two repetitive element subfamilies. The present work is based on a comprehensive methylation analysis of 10 repetitive element subfamilies that were examined in three groups of participants with well-characterized exposure, including steel workers with exposure to PM10 in Study 1; gas station attendants exposed to air benzene in Study 2; and truck drivers exposed to EC in Study 3. We found that effects on DNA methylation of individual repetitive element subfamilies were specific to the exposure type. We selected different ages of repetitive element subfamilies – including old, intermediate, and young subfamilies – based on the time when they appeared in the human genome. We showed exposure-related effects that depended on the subfamily evolutionary age.
The different susceptibility of repetitive element methylation to environmental pollutants may be explained by the sequence variation and GC-content differences between the subfamilies. Subfamilies with older evolutionary age have lower CpG content due to higher substitution rates. As a measure of CpG density, we calculated the ratio between observed and expected CpG content (CpGo/e) for each subfamily (Table 2). Our data confirmed that the subfamily age was inversely correlated with DNA methylation levels in the CpG sites. Also, DNA methylation of those CpG sites was positively correlated with the ratio of CpGo/e. These findings show that older subfamilies have lower CpG density and are prone to have lower DNA methylation. This also supports the concept that each repetitive element family has different patterns of DNA methylation, which might reflect varying degrees of regulation and help explaining the different responses to environmental exposures.
An alternative potential explanation for the exposure-related differences in DNA methylation observed in the present study relates to the genomic position of repetitive elements in the genome. Repetitive element families show different insertional preference in the human genome; for instance, LINE-1s are frequently inserted in AT rich regions, as TTTT/A is the site to prime reverse transcription . Alus and HERVs are more likely inserted into GC rich regions, i.e., in regions near genes or gene-related features such as CpG islands . In some instances, methylation spreads from upstream Alus into the nearby gene-promoter region . Due to the functional relationships between repetitive elements and their surrounding regions, it is possible that differential sensitivity of the insertion regions to environmental exposures could affect DNA methylation of the inserted repetitive element. The methylation difference in this pool of repetitive elements is small (less than 2% in low versus high exposed groups) when expressed in percentage over the total number of cytosines in the considered position. Since we designed subfamily-specific assays, even a small difference in methylation levels might lead to instability of the genomic structure.
Methylation of individual sequences in the repetitive element families LINE-1, Alu, and HERV has been already investigated in relation to environmental exposures such as PM10, black carbon, and persistent organic pollutants [21, 30–37, 44]. However, due to the similarity of the sequences and the difficulty in designing primers for specific subfamilies, most of previous studies have analyzed only one single subfamily (i.e., L1Hs for the LINE-1 family and AluSx for the Alu family) and – to the best of our knowledge – no study has yet investigated multiple subfamilies. In the present study, we found significant associations of DNA methylation of specific repetitive element subfamilies in both the analysis using exposure groups (high vs. low) and in the dose–response analysis using continuous levels of exposure to metal-rich PM10, air benzene, or EC. However, not all the effects on DNA methylation were consistently found in both the group and dose–response analysis. For instance, L1Ta showed a significant difference in highly-exposed steel workers in Study 1, but the group analysis was not confirmed in the dose–response analysis using continuous PM10 levels. Similarly, in the Alu family, DNA methylation of the intermediate-age AluYb8 sequence showed a significant difference in the highly-exposed group of truck drivers in Study 3, which was not confirmed in the dose–response analysis using continuous EC levels. The small number of individuals in each group might explain at least part of these inconsistencies. It is worth noting that, even in the cases with discordance of statistical significance, group and dose–response analysis were concordant in showing similar directions for the exposure-related methylation differences.
Repetitive element subfamilies were inserted in the host human genome at different evolutionary ages. To provide more stable estimates of the general effects of air pollutants, as well as to elucidate the biological bases of the heterogeneity of effects within repetitive element family, we investigated whether the correlations between subfamily methylation and exposures depended on the subfamily evolutionary age. We applied arbitrary numbers at regular intervals of 2, 6, 11, 15, and 20 million years ago (Mya), reflecting a plausible range and spaced intervals of evolutionary ages. We observed that the effects of air pollutants on repetitive element methylation – particular in LINE-1 subfamilies – were significantly affected by the age of subfamilies. The interaction analysis of environmental exposure and ages of repetitive element subfamilies suggested that DNA methylation in older LINE-1 subfamilies might be more vulnerable to environmental exposure than in younger subfamilies.
Our findings are consistent with the hypothesis that exposures activate pollutant-specific biological pathways, which may in turn result in signature differences in DNA methylation in specific repetitive element subfamilies. DNA methyltransferases (DNMTs) play a fundamental role in the generation of DNA methylation by transferring methyl groups from S-adenosyl-methionine (SAM) to the C5 position of the pyrimidine ring of cytosines. DNMTs are environmentally sensitive  and may represent vulnerable targets in the biological process linking pollutant exposures to DNA methylation. Specific DNMT isoforms show different sensitivity to the environment, as potentially each pollutant might target one or a combination the several DNMT isoforms. DNMT isoforms also have different activity in the methylation of individual repetitive element subfamilies . For instance in vitro studies showed that LINE-1 sequences are preferentially methylated by the DNMT3B1, DNMT3B2, and DNMTΔ3B isoforms, which however do not produce any methylation on AluYb8. Taken together, these data indicate that different susceptibility of DNMTs to environmental exposures could modify their subfamily-specific activity on DNA methylation.
The present study has a number of limitations. The small sample size of 40 participants from each of the three studies might have limited the power to detect exposure-related differences. Despite designing PCR primers on highly homologous sequence regions between subfamilies, our assays might have missed some copies of each subfamily due to the sequence variations inherent to repetitive element subfamilies. In addition, because of the general characteristics of sodium-bisulfite conversion, we could not distinguish between CpG to TpG mutation and cytosine methylation in CpG sites. Separate genomic sequencing would be necessary to identify bona-fide cytosine methylation in CpG sites. The selection of repetitive elements in this study was limited to representative sequences with different evolutionary ages. Future studies are needed including larger numbers of subfamilies. Nonetheless, to the best of our knowledge, the present study includes the most comprehensive selection to date of subfamilies ever examined in relation with environmental exposure.
This study has also a number of strengths that support the validity of the results. We conducted a comprehensive DNA methylation analysis of repetitive element subfamilies of different evolutionary ages within three distinct families. We used a highly quantitative bisulfite-PCR-pyrosequencing approach for DNA methylation analysis, which is the gold standard for DNA methylation analyses in short (up to 80–100 bp) sequences. Finally, we evaluated three different airborne pollutants, whose exposure was assessed through directly measured or estimated levels at the personal level. Significant differences in methylation level were found in repetitive element subfamilies that had not yet been analyzed in environmental studies. Our results suggest that previous studies that aimed at evaluating global genomic methylation by just considering specific subfamilies of L1HS and AluSx might have missed significant associations of repetitive element DNA methylation with environmental exposures. Using an augmented panel of repetitive element subfamilies might help to identify novel effects of environmental exposures.
In conclusion, the present study on DNA methylation of 10 repetitive element subfamilies showed family- and subfamily-specific effects in three distinct conditions of exposures to different types of air pollutants. We also showed that sensitivity to environmental exposures is dependent on the evolutionary ages of the repetitive element subfamilies. Our results provide better understanding of the effects of the exposures on methylation of repetitive element subfamilies and may help to elucidate the role of repetitive elements in response to environmental risk factors related to human health and disease.
Study participants and exposure levels
We selected a total of 120 healthy individuals who participated in three previous studies of different types of airborne pollutants. From each of the three studies, we selected two groups of 20 highly-exposed individuals and 20 low-exposed controls matched by age and smoking status. In consideration of the predominance of males in the three original studies and to limit potential confounding by gender, we only sampled male participants. Table 1 shows the characteristics and the exposure levels of the three studies.
In Study 1, the participants were recruited from steel workers in Brescia, Italy . In brief, we selected among workers in a steel production plant 20 steel workers with high exposure to metal-rich particles (Particulate Matter with aerodynamic diameter >10 μm [PM10] ≥ 152.2 μg/m3) and 20 office workers as controls with low exposures (PM10 ≤ 150.0 μg/m3).
In Study 2, we selected 20 gas station attendants exposed to airborne benzene in Milan, Italy as the highly-exposed group (air benzene ≥ 31.2 μg/m3); and 20 office workers as the low-exposed control group (air benzene ≤ 23.0 μg/m3) .
In Study 3, we selected 20 truck drivers with exposure Elemental Carbon (EC), taken as a tracer of traffic particles, in Beijing, China as the highly-exposed group (EC ≥ 16.6 μg/m3); and 20 office workers as the low-exposed control group (EC ≤ 16.1 μg/m3) .
Exposure assessment is described in the Additional file 1: Table S3.
Sample preparation was conducted in all studies using the same standardized procedures. Buffy coat from whole blood was collected from each participant and immediately stored at -80°C until genomic DNA was isolated. Genomic DNA from all participants within each study was isolated with the same batch of reagents in narrow time windows in order to minimize technical and operator variations. The isolated genomic DNA was stored at -80°C for future use. Details on sample collection and DNA isolation for each study have been described in previous publications [30, 31, 49].
DNA methylation analysis
One μg of genomic DNA was treated using the EZ DNA Methylation Kit (Zymo Research, Orange, CA, USA) according to the manufacturer’s protocol. Final elution was performed with 30 μL M-Elution Buffer. Bisulfite-treated DNA was aliquoted and stored at -80°C until ready for use. We performed DNA methylation analyses on bisulfite-treated DNA using highly quantitative analysis based on PCR-pyrosequencing. The PCR and pyrosequencing primer sequences for L1HS, AluSx, and AluYb8 have been previously published by Yang et al.  and by Choi et al. . We developed additional assays specific for three LINE-1 subfamilies (L1PA5, L1PA2, and L1Ta), for AluYd6 subfamily, and for three HERV subfamilies (MLT1D, ERV1, and ERV9) (see Additional file 1 for primer sequences and PCR conditions). A minimum number of 1 CpG and a maximum of 5 CpGs were evaluated in each assay. PCR amplification was performed at standard conditions using the GoTaq® Hot Start Polymerase (Promega, Madison, WI).
We used a PSQ Q96 MD pyrosequencing System (QIAGEN, Valencia, CA), as previously described . Each sample was tested two times for each assay to confirm reproducibility. As a quality control check to estimate bisulfite conversion efficiency, we placed duplicate genomic DNA samples on each bisulfite conversion plate to estimate the internal plate variation of bisulfite conversion and the pyrosequencing reaction. We also added universal PCR products amplified from cell-line DNA on each pyrosequencing plate to check run-to-run and plate-to-plate variation. In addition, the Pyrogram peak pattern from every sample was visually inspected to confirm the quality of the reaction.
Characteristics of the repetitive element subfamilies
The repetitive element evolutionary age is defined based on the estimated time when each subfamily appeared in the human genome. We identified an evolutionary age (expressed in Mya [million years ago]) for each repetitive element subfamily based on information available in previous literature [41–43, 51, 52] (Table 2). As a measure of CpG density we calculated the ratio between observed and expected CpG content (CpGo/e) for each subfamily. The CpG observed/expected (CpG o/e) ratio is calculated by formula; ((Num of CpG/(Num of C × Num of G)) × Total number of nucleotides in the sequence)  (Table 2).
We used mixed-effect regression models to evaluate the effects of the exposures on DNA methylation levels of each subfamily, as previously reported . This approach yields a global estimate of the effect on multiple CpGs within each subfamily sequence by modeling correlated data in adjacent CpG sites within each sequence, as well as the measures from duplicate pyrosequencing runs. Mixed-effect models have the advantage over standard methods of using the entirety of the information in the data, thus maximizing statistical power by distinguishing different sources of variance. See Additional file 1 for the detailed description of the models used.
This work has been supported in part by the HSPH-NIEHS Center for environmental health (ES000002); and by related work in the NIEHS grants ES021733 and ES021895. The Beijing air pollution study has been supported by NIEHS grants ES020010 and ES020984.
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