Ultrasmall superparamagnetic iron oxide nanoparticles acutely promote thrombosis and cardiac oxidative stress and DNA damage in mice
© Nemmar et al. 2016
Received: 23 November 2015
Accepted: 18 April 2016
Published: 30 April 2016
Ultrasmall superparamagnetic iron oxide nanoparticles (USPIO) are being developed for several biomedical applications including drug delivery and imaging. However, little is known about their possible adverse effects on thrombosis and cardiac oxidative and DNA damage.
Presently, we investigated the acute (1 h) effect of intravenously (i.v.) administered USPIO in mice (0.4, 2 and 10 μg/kg). Diesel exhaust particles (DEP; 400 μg/kg) were used as positive control.
USPIO induced a prothrombotic effect in pial arterioles and venules in vivo and increased the plasma plasminogen activator inhibitor-1 (PAI-1). Both thrombogenicity and PAI-1 concentration were increased by DEP. The direct addition of USPIO (0.008, 0.04 and 0.2 μg/ml) to untreated mouse blood dose-dependently induced in vitro platelet aggregation. USPIO caused a shortening of activated partial thromboplastin time (aPTT) and prothrombin time (PT). Similarly, DEP administration (1 μg/ml) triggered platelet aggregation in vitro in whole blood. DEP also reduced PT and aPTT. The plasma levels of creatine phosphokinase-MB isoenzyme (CK-MB), lactate dehydrogenase (LDH) and troponin-I were increased by USPIO. DEP induced a significant increase of CK-MB, LDH and troponin I levels in plasma. The cardiac levels of markers of oxidative stress including lipid peroxidation, reactive oxygen species and superoxide dismutase activity were increased by USPIO. Moreover, USPIO caused DNA damage in the heart. Likewise, DEP increased the markers of oxidative stress and induced DNA damage in the heart.
We conclude that acute i.v. administration of USPIO caused thrombosis and cardiac oxidative stress and DNA damage. These findings provide novel insight into the pathophysiological effects of USPIO on cardiovascular homeostasis, and highlight the need for a thorough evaluation of their toxicity.
Nanotechnology is a broad interdisciplinary area that involves biochemistry, physics, biology, materials science, electrical engineering and more [1, 2]. Nanotechnology holds great promise in numerous fields, including medicine. In the latter field, it has developed rapidly to create a new application of nanotechnology in medicine known as nanomedicine which aims at the improvement of targeted drug delivery, better diagnostic and imaging techniques [1, 2]. Nanomedicine develops potential therapies designed to diagnose and treat specific diseases [1, 2]. However, concerns have been expressed about the possible adverse effects of nanomaterials [1–3].
Ultrasmall superparamagnetic iron oxide nanoparticles (USPIO, < 50 nm) have an iron oxide core that is monomer or polymer-coated/stabilized. They possess highly reactive surface, uniform particle size distribution, good suspension and the possibility of additional coating modification by conjugating with a drug [4–6]. USPIO are being developed for drug delivery and neuroimaging and imaging of tumours and metastases in the liver, spleen and bone marrow, and perfusion imaging of atherosclerotic plaque and thrombosis [4–7] However, the existing information regarding the toxicity of USPIO is rather limited, and little attention has been paid to their potential in vivo toxicity . Moreover, most information on their in vivo toxicity has been derived from studies that were not specifically designed to evaluate their toxicity .
It is well-established that particulate air pollution and some engineered nanoparticles (such as silica) cause prothrombotic effects [9–11]. Since USPIO are being administered directly into the circulation following intravenous injection in perfusion imaging of thrombosis , we hypothesized that these nanoparticles can plausibly interact with the vasculature and circulating cells such as platelets and could, possibly, aggravate thrombosis. Moreover, such administered nanoparticles can also reach the heart and cause oxidative stress and DNA damage. Therefore, the aim of this study was to investigate the acute (1 h) effect of intravenously (i.v.) administered USPIO in mice (0.4, 2 and 10 μg/kg) on thrombosis in vivo and in vitro and cardiac oxidative stress and DNA damage. Appropriate positive control particles were used in the present study, i.e. diesel exhaust particles (DEP) which are known to cause thrombosis, cardiotoxicity and oxidative stress [12–14]. The dose of DEP tested (400 μg/kg or 0.4 mg/kg) is comparable to that used recently by Tabor et al. for i.v. injection (0.5 mg/kg) in rats .
USPIO and DEP
As described by the manufacturer (Sigma, St. Louis, MO, USA), the USPIO (magnetite, Fe3O4) used in the present study have a particle size ranged between 4 and 6 nm (diameter 5 nm, by transmission electron microscopy), density 5 mg/mL in H2O [includes total weight of nanoparticles plus <1.0 % modified short chain polyethylene glycol (PEG) stabilizing ligands], 1 mg/ml Fe and magnetization >25 emu/g, at 4500Oe.
To confirm their size, we performed transmission electron microscopy analysis of the USPIO. Thus, droplets (10 μL) of a suspension of 0.1 mg of USPIO in 500 μL were placed on matured formvar/carbon film for 30 s. The samples were then drained and inverted onto droplets of ultrapure water for 1 h before being drained, dried, and examined in a Philips CM10 Transmission Electron Microscope.
The DEP were obtained from the National Institute of Standards and Technology (NIST, Gaithersburg, MD, USA), and were suspended in sterile saline (NaCl 0.9 %). These particles were previously analysed by transmission electron microscopy, and shown to have a substantial amount of ultrafine (nano) sized particle aggregates, and larger particle aggregates .
The endotoxin concentration in the USPIO solution, DEP and saline used was quantified, as described by the manufacturer, by chromogenic Limulus Amebocyte Lysate (Pierce, Rockford, IL) test. The concentrations were lower than the detection limit (0.1 EU/ml) in the saline, and USPIO and DEP solutions.
Animals and i.v. administration of USPIO or DEP
This project was reviewed and approved by our Institutional Review Board and experiments were performed in accordance with protocols approved by the Institutional Animal Care and Research Advisory Committee.
USPIO or DEP were suspended in normal saline (NaCl 0.9 %). To minimize aggregation, particle suspensions were always sonicated (Clifton Ultrasonic Bath, Clifton, New Jersey, USA) for 15 min and vortexed before their dilution and prior to i.v. administration.
BALB/C mice (Taconic Farms Inc., Germantown, NY, USA) weighing 20–25 g were housed in light (12-h light:12-h dark cycle) and temperature-controlled (22 ± 1 °C) rooms. They had access to commercial laboratory chow and tap water ad libitum.
The mice were anesthetized with urethane (1 mg/g body weight, i.p.), and 2 F catheter (Portex, Hythe, UK) was inserted in the right jugular vein. Either USPIO suspensions (0.4, 2 and 10 μg/kg) or DEP (400 μg/kg) or saline-only were i.v. injected (100 μl), and 1 h later various cardiovascular parameters were assessed.
Experimental thrombosis model in pial arterioles and venules
The assessment of thrombosis has been performed according to a previously described technique [16–19]. Briefly, the trachea was intubated after induction of anaesthesia with urethane (1 mg/g body weight, i.p.), and a 2 F venous catheter (Portex, Hythe, UK) was inserted in the right jugular vein for the administration of fluorescein (Sigma, St. Louis, MO, USA). After that, a craniotomy was first performed on the left side, using a microdrill, and the dura was stripped open. Only untraumatised preparations were used, and those showing trauma to either microvessels or underlying brain tissue were discarded. The animals were then placed on the stage of a fluorescence microscope (Olympus, Melville, NY, USA) attached to a camera and DVD recorder. A heating mat was placed under the mice and body temperature was raised to 37 °C, as monitored by a rectal thermoprobe connected to a temperature reader (Physitemp Instruments, NJ, USA). The cranial preparation was moistened continuously with artificial cerebrospinal fluid of the following composition (mM): NaCl 124, KCl 5, NaH2PO4 3, CaCl2 2.5, MgSO4.4, NaHCO3 23 and glucose 10, pH 7.3–7.4. A field containing arterioles and venules 15–20 μm in diameter was chosen. Such a field was taped prior to and during the photochemical insult, which was carried out by injecting fluorescein (0.1 ml/mouse of 5 % solution) via the jugular vein, which was allowed to circulate for 30–40 s. The cranial preparation was then exposed to stabilized mercury light. The combination produces endothelium injury of the arterioles and venules. This, in turn, causes platelets to adhere at the site of endothelial damage and then aggregate. Platelets aggregate and the thrombus forms and grows in size until complete vascular occlusion. The time, in seconds, was measured from the photochemical injury until full vascular occlusion (time to flow stop) in venules. One hour before the administration of fluorescein, 100 μl of saline (control) or saline containing either USPIO or DEP was administered via the jugular vein. The animals were euthanized at the end of the recording by an overdose of anaesthesia.
Blood collection and analysis
In separate experiments, new mice were anesthetized and were given i.v. saline (control) or saline containing either USPIO or DEP as described above. One h later, the animals’ blood was drawn from the inferior vena cava in EDTA (4 %) and centrifuged at 4 °C for 15 min at 3000 rpm, and the plasma samples obtained were stored at−80 °C until further analysis.
Determination of systemic markers of fibrinolysis and myocardial membrane damage
The concentration of plasminogen activator inhibitor-1 (PAI-1) (Molecular Innovation, Southfield, MI, USA) was determined using ELISA Kit. Creatine phosphokinase-MB isoenzyme (CK-MB), troponin-I and lactate dehydrogenase (LDH) were measured spectrophotometrically using kits (Roche, Basel, Switzerland).
Measurement of cardiac markers of oxidative stress
The hearts obtained from the animals used above were quickly rinsed with ice-cold PBS (pH 7.4) before homogenization in 0.1 M phosphate buffer pH 7.4 containing 0.15 M KCl, 0.1 mM EDTA, 1 mM DTT and 0.1 mM phenylmethylsulfonylfluoride at 4 °C. The homogenates were centrifuged for 20 min at 14,000 rpm to remove cellular debris and supernatants were used for further analysis. Protein content was measured by Bradford’s method as described before [20–22].
Measurement of reactive oxygen species (ROS): ROS were measured in the whole cardiac tissue homogenates which were obtained as described above using 2’, 7’- Dichlorofluorescein diacetate (DCFDA; Molecular Probes, Eugene, OR, USA) as a fluorescent probe as described before [20–22]. The results were normalized as ROS produced per mg of protein.
NADPH-dependent membrane LPO in the heart homogenate was determined using TBARS kit purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Measurement of superoxide dismutase (SOD) was performed by an ELISA technique with commercially available kit (Cayman Chemicals, Michigan, USA).
In vitro platelet aggregation in mouse whole blood
The platelet aggregation assay in whole blood was performed, with slight modification, as described before . After anaesthesia, blood from untreated mice was withdrawn from the inferior vena cava and placed in citrate (3.2 %), and 100-μl aliquots were added to the well of a Merlin coagulometer (MC 1 VET; Merlin, Lemgo, Germany). The blood samples were incubated at 37.2 °C with either saline (control) or USPIO (0.008, 0.04 and 0.2 μg/ml) or DEP (1 μg/ml) for 3 min, and then stirred for another 3 min. At the end of this period, 25-μl samples were removed and fixed on ice in 225 ml cellFix (Becton Dickinson). After fixation, single platelets were counted in a VET ABX Micros with a mouse card (ABX, Montpellier, France). The degree of platelet aggregation following USPIO or DEP exposure was expressed as a ℅ of control (saline-treated blood).
In vitro measurement of prothrombin time (PT) and activated partial thromboplastin time (aPTT) in plasma
One hour following either saline or USPIO or DEP administration, blood was withdrawn from each mouse, as described above. The PT was measured [24–26] on freshly collected, platelet-poor plasma with human, relipidated, recombinant thromboplastin (Recombiplastin; Instrumentation Laboratory, Orangeburg, NY, USA) in combination with a Merlin coagulometer MC 1 VET (Merlin, Lemgo, Germany). The aPTT was measured [24–26] with automated aPTT reagent (BioMerieux, Durham, NC, USA) using a Merlin coagulometer MC 1 VET (Merlin, Lemgo, Germany). Normal plasma used as reference for both the PT and aPTT was prepared by pooling equal portions of platelet-poor plasmas from the blood of six untreated mice.
DNA damage assessment in the heart by COMET assay
Immediately after sacrifice, the heart was removed from each animal. Single-cell suspensions of the different hearts were obtained according to the method described by de Souza et al.. Each collected organ was washed in a chilled medium (RPMI 1640, 15 % DMSO, 1.8 % (w/v) NaCl). The heart tissues were placed in 1.5 ml medium and chopped finely into pieces in a Petri dish using scissors. The pieces were allowed to settle and the supernatant was collected in a 15 ml tube. The obtained cell suspension was centrifuged at 1000 rpm for 5 min at 40 C. The supernatant was discarded and the pellets were resuspended in 0.5 ml of the medium. The cell suspensions were mixed with low melting point agarose solution (0.65 %) and spread onto agarose (1.5 %)–precoated microscope slides. For each treatment five slides were prepared, which were incubated in ice cold lysis buffer (2.5 M NaCl, 10 mM Tris, 100 mM EDTA, 1 % Triton X-100 and 10 % DMSO) at 40 C for at least 1 h to remove the cell membranes. After the incubation, slides were placed in a horizontal electrophoresis unit and incubated in electrophoresis buffer (0.2 M EDTA, 5 M NaCl, pH 10) for 20 min for DNA unwinding and the expression of alkali labile sites. Then, electrophoresis was conducted for 20 min at 25 V and 300 mA. After that, the slides were neutralized with Tris buffer (0.4 M Trizma base, pH 7.5) for 5 min and washed with methanol. Then the slides were stained with propidium iodide, as previously described [28, 29]. All these steps were performed in darkness to prevent additional DNA damage. The slides were mounted on a fluorescent microscope and cell scoring was performed. Fifty cells from each treatment were scored and analyzed for DNA migration and the average of the five slides from each group was calculated. The measurement of length of the DNA migration (i.e. diameter of the nucleus plus migrated DNA) was calculated using image analysis Axiovision 3.1 software (Carl Zeiss, Canada) [29, 30].
All statistical analyses were performed with GraphPad Prism Software version 5 (San Diego, CA, USA). To determine whether parameters were normally distributed, the Kolmogorov–Smirnov statistic normality test was applied. Comparisons between groups were performed by one way analysis of variance (ANOVA), followed by Newman-Keuls multiple-range tests. Data which were not normally distributed were analyzed with Kruskal-Wallis test followed by Dunn’s multiple comparison test. P-values <0.05 are considered as significant.
Transmission electron microscopy analysis of USPIO
Effect of USPIO or DEP on photochemically-induced thrombosis in pial arterioles and venules
Effect of USPIO or DEP on PAI-1 concentration in plasma
In vitro effect of USPIO or DEP on platelet aggregation in whole blood
Effect of USPIO or DEP on PT and aPTT
In comparison with the value obtained for the control group, the i.v. administration of USPIO caused a significant shortening of PT. The level of significance was achieved at 0.4 μg/kg (P < 0.01), 2 μg/ml (P < 0.001) and 10 μg/ml (P < 0.01) (Fig. 5a). Similarly, the administration of USPIO induced a significant shortening of aPTT at 0.4 μg/ml (P < 0.01), 2 μg/ml (P < 0.01) and 10 μg/ml (P < 0.01) (Fig. 5b). DEP significantly reduced the PT (P < 0.001) and aPTT (P < 0.001).
Effect of USPIO or DEP on plasma activities of LDH, CK-MB and troponin-I
Effect of USPIO or DEP on cardiac oxidative stress
Effect of USPIO on cardiac DNA damage
We showed here that acute (1 h) i.v. administration of USPIO caused prothrombotic events in pial arterioles and venules in vivo, platelet aggregation in whole blood in vitro and shortening of PT and aPTT. The concentration of PAI-1 was increased by USPIO. Markers of myocardial membrane damage in plasma including CK-MB, LDH and troponin-I were significantly increased by USPIO. Moreover, USPIO induced oxidative stress in the heart evidenced by increase of LPO, ROS and SOD, and caused cardiac DNA damage. All the aforementioned endpoints were increased by the administration of the positive control particles, namely DEP.
Commercially available USPIO are surface coated with materials such as silicon, dextran, citrate and PEGylated starch and are mainly used as contrast agents in target organs . In the present study, we tested 5 nm USPIO with PEG stabilizing ligands. PEG has been broadly utilised in the formulation of nanoparticles for biomedical applications, both because of its biocompatibility and its effectiveness in masking nanoparticles from opsonins . These nanoparticles were administered i.v. to the mice. This route of exposure imitates their potential use in clinical settings . Moreover, their small size allow them to cross the capillary beds and gain access to various organs such as lung, brain kidney, spleen, liver and heart . Previously, when dextran-coated superparamagnetic iron oxide nanoparticles were examined as a contrast agent in subjects, their adverse effects (headache, back pain, vasodilatation, and urticarial) were reported to be mild or moderate in severity and of short duration . A dose of 2.6 mg of iron per kg was applied . In experimental arthritis model in mice, doses of 3.3 mg Fe/kg or 33.3 mg Fe/kg were injected i.v. . The doses of USPIO (0.4, 2 and 10 μg per kg) used in our study in mice have low iron content of 0.0004, 0.002 and 0.01 μg Fe per kg, respectively. However, the amount of iron injected is not the sole parameter that determines the toxicity of nanoparticles in general [3, 35]. In fact, it is well established that nanoparticles have unique features including their very small size, large surface area and cellular penetration capacity which are responsible for their specific biological responses that differ from larger particle of similar chemical composition [3, 35, 36]. Recently, it has been shown that i.v. injected polyacrylic acid coated γ-Fe2O3 nanoparticles (10 mg/kg) in healthy BALB/cJ mice induced a decrease in the mean arterial blood pressure (MAP) associated with a decreased contractility of small mesenteric arteries . In anaesthetized pigs, it has been demonstrated that dimercaptosuccinic acid coated superparamagnetic iron oxide nanoparticles (MF66-labelled 12 nm, core nominal diameter and OD15 15 nm); at 0.5, or 2.0 mg/kg injected i.v. induced a transient yet significant decrease in MAP . Particles remained in the circulation for up to 30 min and were accumulated in the liver, spleen and lungs . With regard to human exposure situation, pharmacokinetic data related to the liposome-encapsulated doxorubicin nanoparticles, used in cancer therapy, reported plasma concentrations of more than 10 μg/ml . The last concentration is much higher than that used in the present in vivo study. In fact, the highest dose of nanoparticles used here which is 10 μg/kg (0.25 μg/mouse of 25 g) would correspond to approximately 0.16 μg/ml, assuming a circulating mouse blood volume of 1.5 ml. One of the reasons why we wanted to use lower dose of USPIO in our study is because we wanted to test the possible exacerbating effect of USPIO on an in vivo photochemical injury model of thrombosis in the pial cerebral arterioles and venules. In clinical settings, patients who will be potentially injected with these nanoparticles, for diagnosis or treatment purposes, will likely have pre-existing diseases (e.g. cardiovascular diseases). Therefore, we can speculate that those patients may conceivably exhibit increased susceptibility to adverse cardiovascular effect of USPIO as compared with healthy peoples. In this sense, the minor photochemical trauma to endothelium used in our experiments may have pathophysiological relevance . Superficial erosion, or microscopic areas of desquamation of endothelial cells, occurs frequently in both humans and in animals with experimentally-induced atherosclerosis . The doses of DEP used in vivo (400 μg/kg or 0.4 mg/kg) and in vitro (1 μg/ml) are similar to those used recently to study the prothrombotic effects of DEP in vivo and in vitro [12, 13]. Our data show that the i.v. administration of USPIO for 1 h caused a significant and dose-dependent shortening of the thrombotic occlusion time, showing that USPIO have prothrombotic effect. Several previous studies have reported the use of USPIO for perfusion imaging of atherosclerotic plaque and thrombosis . For example, in a recent study, USPIO were injected i.v. at different time points after photothrombotic infarction in mice, and it was possible to follow vessel occlusion in vivo by USPIO-enhanced high-field MRI . It has been reported that i.v. administration of polystyrene nanoparticles in hamsters, rats and mice, and DEP in rats and mice cause in vivo thrombogenicity [15, 36, 42–44]. Nevertheless, the possible prothrombotic effect of USPIO has, as far as we are aware, never been reported so far.
In the present study, we also found a significant increase of circulating PAI-1 following the administration of USPIO. PAI-1 is the most potent endogenous inhibitor of fibrinolysis and is involved in the pathogenesis of several cardiovascular diseases [45, 46]. An increase of PAI-1 has been observed following exposure to either DEP  or silica nanoparticles .
Our model of thrombosis depends on platelet activation and aggregation . Consequently, we wanted to verify whether and to what extent can USPIO directly activate platelet and cause their aggregation in whole blood in vitro. Since the highest dose of nanoparticles tested, presently, in vivo (10 μg/kg or 0.25 μg/mouse) would correspond to approximately 0.16 μg/ml blood, we selected to test in vitro concentrations which are relevant to our in vivo study, i.e. 0.2 μg/ml being the highest concentration and 2 lower ones (0.04 and 0.008 μg/ml). Our data show that low concentrations of USPIO cause a significant and dose-dependent proaggregatory effect, confirming the in vivo prothrombotic findings. Although USPIO are developed for i.v. administration, and hence, the study of their interaction with circulating cells such as platelets is critical, little is known about their potential effect on platelet aggregation. Several studies focused on designing functionalized USPIO to target activated platelets for the study atheroma pathogenesis [7, 48]. However, other types of particles such silica, polystyrene, DEP or TiO2 have previously been reported to induce platelet aggregation in vitro [11, 23, 36, 44, 49, 50]. Furthermore, we demonstrated the activation of PT and aPTT in plasma of mice in vivo administered with various doses of USPIO. This effect reflects hypercoagulability caused by USPIO and confirm the in vivo and in vitro prothrombotic effects of these nanoparticles. High concentration of maghemite nanoparticles bioferofluids (0.38 mg/ml Fe2O3) has been reported to shorten PT but, on the other hand, it caused prolongation of aPTT . Here, we also found that DEP caused a shortening of PT and aPTT, confirming our earlier findings . Our data showed significant increments in cardiac enzyme levels of LDH, CK-MB and troponin-I (at 10 μg/kg), which may be attributed to the myocardial membrane damage produced by USPIO and the leakage of these enzymes into blood . To gain more insight into the cardiac toxicity of USPIO, we assessed the effect of these nanoparticles on oxidative stress and DNA damage in the heart tissue. Oxidative stress is a key pathological process in a variety of pathophysiological conditions affecting various organs such as the lung, heart, kidney or liver and is characterized by the formation of a wide range of ROS, which can cause severe DNA, protein, and lipid damage leading to cellular dysfunction and death . Iron oxide nanoparticles found either in the environment or in biomedical settings are readily taken up by the cells and cause the generation of free radicals leading to oxidative stress [8, 54]. It has been shown that even stabilized iron oxide particles with coatings such as dextran or citric acid may cause oxidative stress . More recently, to mitigate oxidative stress-induced by iron oxide nanoparticles, antioxidant polymer poly(trolox) was formulated into nanoparticles coated with an antibody directed towards platelet endothelial cell adhesion molecule-1 . These active targeting nanoparticles have shown to adhere to human umbilical vein endothelial cells, internalize, and reduce oxidative stress induced by iron oxide particles . Nevertheless, the effect of USPIO on oxidative stress and DNA damage in the heart has never been reported so far. Our data show a significant increase in LPO and ROS in the heart. Moreover, we found a significant increase of the antioxidant SOD in the heart. This indicates that the development of oxidative stress is accompanied by an adaptive response that counterbalances the potentially damaging activity of oxygen free radicals by antioxidant defense mechanisms. Increase of SOD following exposure to silica nanoparticles has been recently reported in mouse erythrocytes  and in the heart of mice exposed to cigarette smoke . Likewise, in the present study, DEP induced a significant increase of all the markers of oxidative stress studied. For evaluating genetic damage, the gel electrophoresis of a single cell (Comet assay) was used to assess DNA damage in the heart following USPIO administration. Our data show a significant DNA damage in the heart after the acute administration of USPIO. DEP caused similar effect. We speculate that ROS generation and oxidative stress caused by USPIO induced DNA damage in the heart. Such an effect has not been reported before. An in vitro study showed that iron oxide nanoparticles caused DNA damage and apoptosis through ROS generation in human breast cancer cell line . A recent study in rats using high doses of Fe2O3-30 nm nanoparticles (500–2000 mg/kg) given orally did not assess DNA damage in heart but showed no genotoxicity in circulating leukocytes between 6 h and 72 h post-exposure .
We conclude that acute i.v. administration of USPIO exerts procoagulatory effect in vivo and in vitro and cause cardiac oxidative stress and DNA damage. These findings provide novel insight into the pathophysiological effects of USPIO on cardiovascular system, and highlight the need for a comprehensive assessment of their toxicity before their clinical use. Additional studies are needed to investigate the effect of various coating such as dextran on the toxicity of USPIO and the mechanisms underlying the effects of USPIO on platelet activation in human blood.
This work was supported by funds of the College of Medicine and Health Sciences grant (31 M215), and United Arab Emirates University UPAR (31 M189) and center-based interdisciplinary (31R052) grants.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Hoet PHM, Nemmar A, Nemery B. Health impact of nanomaterials? Nat Biotechnol. 2004;22:19.View ArticlePubMedGoogle Scholar
- Oberdorster G. Safety assessment for nanotechnology and nanomedicine: concepts of nanotoxicology. J Intern Med. 2010;267:89–105.View ArticlePubMedGoogle Scholar
- Nemmar A, Holme JA, Rosas I, Schwarze PE, Alfaro-Moreno E. Recent advances in particulate matter and nanoparticle toxicology: a review of the in vivo and in vitro studies. Biomed Res Int. 2013;2013:279371.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhao X, Zhao H, Chen Z, Lan M. Ultrasmall superparamagnetic iron oxide nanoparticles for magnetic resonance imaging contrast agent. J Nanosci Nanotechnol. 2014;14:210–20.View ArticlePubMedGoogle Scholar
- Usman A, Sadat U, Patterson AJ, Tang TY, Varty K, Boyle JR, et al. Use of ultrasmall superparamagnetic iron oxide particles for imaging carotid atherosclerosis. Nanomedicine (Lond). 2015. [Epub ahead of print].Google Scholar
- Suzuki M, Bachelet-Violette L, Rouzet F, Beilvert A, Autret G, Maire M, et al. Ultrasmall superparamagnetic iron oxide nanoparticles coated with fucoidan for molecular MRI of intraluminal thrombus. Nanomedicine (Lond). 2015;10:73–87.View ArticleGoogle Scholar
- Ittrich H, Peldschus K, Raabe N, Kaul M, Adam G. Superparamagnetic iron oxide nanoparticles in biomedicine: applications and developments in diagnostics and therapy. Röfo. 2013;185:1149–66.PubMedGoogle Scholar
- Singh N, Jenkins GJ, Asadi R, Doak SH: Potential toxicity of superparamagnetic iron oxide nanoparticles (SPION). Nano Rev. 2010, 1. doi: 10.3402/nano.v1i0.5358.Google Scholar
- Nemmar A, Hoet PH, Dinsdale D, Vermylen J, Hoylaerts MF, Nemery B. Diesel exhaust particles in lung acutely enhance experimental peripheral thrombosis. Circulation. 2003;107:1202–8.View ArticlePubMedGoogle Scholar
- Mutlu GM, Green D, Bellmeyer A, Baker CM, Burgess Z, Rajamannan N, et al. Ambient particulate matter accelerates coagulation via an IL-6-dependent pathway. J Clin Invest. 2007;117:2952–61.View ArticlePubMedPubMed CentralGoogle Scholar
- Nemmar A, Albarwani S, Beegam S, Yuvaraju P, Yasin J, Attoub S, et al. Amorphous silica nanoparticles impair vascular homeostasis and induce systemic inflammation. Int J Nanomedicine. 2014;9:2779–89.View ArticlePubMedPubMed CentralGoogle Scholar
- Tabor CM, Shaw CA, Robertson S, Miller MR, Duffin R, Donaldson K, et al. Platelet activation independent of pulmonary inflammation contributes to diesel exhaust particulate-induced promotion of arterial thrombosis. Part Fibre Toxicol. 2016;13:6.View ArticlePubMedPubMed CentralGoogle Scholar
- Nemmar A, Al Dhaheri R, Alamiri J, Al Hefeiti S, Al Saedi H, Beegam S, et al. Diesel exhaust particles induce impairment of vascular and cardiac homeostasis in mice: ameliorative effect of emodin. Cell Physiol Biochem. 2015;36:1517–26.View ArticlePubMedGoogle Scholar
- Lucking AJ, Lundback M, Mills NL, Faratian D, Barath SL, Pourazar J, et al. Diesel exhaust inhalation increases thrombus formation in man. Eur Heart J. 2008;29:3043–51.View ArticlePubMedGoogle Scholar
- Nemmar A, Al Maskari S, Ali BH, Al Amri IS. Cardiovascular and lung inflammatory effects induced by systemically administered diesel exhaust particles in rats. Am J Physiol Lung Cell Mol Physiol. 2007;292:L664–70.View ArticlePubMedGoogle Scholar
- Nemmar A, Al Salam S, Dhanasekaran S, Sudhadevi M, Ali BH. Pulmonary exposure to diesel exhaust particles promotes cerebral microvessel thrombosis: protective effect of a cysteine prodrug l-2-oxothiazolidine-4-carboxylic acid. Toxicology. 2009;263:84–92.View ArticlePubMedGoogle Scholar
- Nemmar A, Al-Salam S, Zia S, Marzouqi F, Al-Dhaheri A, Subramaniyan D, et al. Contrasting actions of diesel exhaust particles on the pulmonary and cardiovascular systems and the effects of thymoquinone. Br J Pharmacol. 2011;164:1871–82.View ArticlePubMedPubMed CentralGoogle Scholar
- Nemmar A, Yuvaraju P, Beegam S, Ali BH. Short-term nose-only water-pipe (shisha) smoking exposure accelerates coagulation and causes cardiac inflammation and oxidative stress in mice. Cell Physiol Biochem. 2015;35:829–40.View ArticlePubMedGoogle Scholar
- Nemmar A, Subramaniyan D, Yasin J, Ali BH. Impact of experimental type 1 diabetes mellitus on systemic and coagulation vulnerability in mice acutely exposed to diesel exhaust particles. Part Fibre Toxicol. 2013;10:14.View ArticlePubMedPubMed CentralGoogle Scholar
- Nemmar A, Raza H, Subramaniyan D, John A, Elwasila M, Ali BH, et al. Evaluation of the pulmonary effects of short-term nose-only cigarette smoke exposure in mice. Exp Biol Med (Maywood). 2012;237:1449–56.View ArticleGoogle Scholar
- Nemmar A, Raza H, Subramaniyan D, Yasin J, John A, Ali BH, et al. Short-term systemic effects of nose-only cigarette smoke exposure in mice: role of oxidative stress. Cell Physiol Biochem. 2013;31:15–24.View ArticlePubMedGoogle Scholar
- Nemmar A, Al-Salam S, Yuvaraju P, Beegam S, Ali BH. Emodin mitigates diesel exhaust particles-induced increase in airway resistance, inflammation and oxidative stress in mice. Respir Physiol Neurobiol. 2015;215: 51-57.Google Scholar
- Nemmar A, Melghit K, Ali BH. The acute proinflammatory and prothrombotic effects of pulmonary exposure to rutile TiO2 nanorods in rats. Exp Biol Med (Maywood). 2008;233:610–9.View ArticleGoogle Scholar
- Nemmar A, Al Salam S, Zia S, Dhanasekaran S, Shudadevi M, Ali BH. Time-course effects of systemically administered diesel exhaust particles in rats. Toxicol Lett. 2010;194:58–65.View ArticlePubMedGoogle Scholar
- Nemmar A, Zia S, Subramaniyan D, Fahim MA, Ali BH. Exacerbation of thrombotic events by diesel exhaust particle in mouse model of hypertension. Toxicology. 2011;285:39–45.View ArticlePubMedGoogle Scholar
- Nemmar A, Yuvaraju P, Beegam S, John A, Raza H, Ali BH. Cardiovascular effects of nose-only water-pipe smoking exposure in mice. Am J Physiol Heart Circ Physiol. 2013;305:H740–6.View ArticlePubMedGoogle Scholar
- de Souza MF, Goncales TA, Steinmetz A, Moura DJ, Saffi J, Gomez R, et al. Cocaine induces DNA damage in distinct brain areas of female rats under different hormonal conditions. Clin Exp Pharmacol Physiol. 2014;41:265–9.View ArticlePubMedGoogle Scholar
- Olive PL, Banath JP, Fjell CD. DNA strand breakage and DNA structure influence staining with propidium iodide using the alkaline comet assay. Cytometry. 1994;16:305–12.View ArticlePubMedGoogle Scholar
- Nemmar A, Al-Salam S, Yuvaraju P, Beegam S, Yasin J, Ali BH. Chronic exposure to water-pipe smoke induces alveolar enlargement, DNA damage and impairment of lung function. Cell Physiol Biochem. 2016;38:982–92.View ArticlePubMedGoogle Scholar
- Hartmann A, Speit G. The contribution of cytotoxicity to DNA-effects in the single cell gel test (comet assay). Toxicol Lett. 1997;90:183–8.View ArticlePubMedGoogle Scholar
- Owens III DE, Peppas NA. Opsonization, biodistribution, and pharmacokinetics of polymeric nanoparticles. Int J Pharm. 2006;307:93–102.View ArticlePubMedGoogle Scholar
- Elias A, Tsourkas A: Imaging circulating cells and lymphoid tissues with iron oxide nanoparticles. Hematology Am Soc Hematol Educ Program 2009, 720–726. doi: 10.1182/asheducation-2009.1.720.Google Scholar
- Anzai Y, Piccoli CW, Outwater EK, Stanford W, Bluemke DA, Nurenberg P, et al. Evaluation of neck and body metastases to nodes with ferumoxtran 10-enhanced MR imaging: phase III safety and efficacy study. Radiology. 2003;228:777–88.View ArticlePubMedGoogle Scholar
- Vermeij EA, Koenders MI, Bennink MB, Crowe LA, Maurizi L, Vallee JP, et al. The in-vivo use of superparamagnetic iron oxide nanoparticles to detect inflammation elicits a cytokine response but does not aggravate experimental arthritis. PLoS One. 2015;10:e0126687.View ArticlePubMedPubMed CentralGoogle Scholar
- Napierska D, Thomassen LC, Lison D, Martens JA, Hoet PH. The nanosilica hazard: another variable entity. Part Fibre Toxicol. 2010;7:39.View ArticlePubMedPubMed CentralGoogle Scholar
- Smyth E, Solomon A, Vydyanath A, Luther PK, Pitchford S, Tetley TD, et al. Induction and enhancement of platelet aggregation in vitro and in vivo by model polystyrene nanoparticles. Nanotoxicology. 2015;9:356–64.View ArticlePubMedGoogle Scholar
- Iversen NK, Frische S, Thomsen K, Laustsen C, Pedersen M, Hansen PB, et al. Superparamagnetic iron oxide polyacrylic acid coated gamma-Fe2O3 nanoparticles do not affect kidney function but cause acute effect on the cardiovascular function in healthy mice. Toxicol Appl Pharmacol. 2013;266:276–88.View ArticlePubMedGoogle Scholar
- Edge D, Shortt CM, Gobbo OL, Teughels S, Prina-Mello A, Volkov Y, et al. Pharmacokinetics and bio-distribution of novel super paramagnetic iron oxide nanoparticles (SPIONs) in the anaesthetized pig. Clin Exp Pharmacol Physiol. 2015;43(3):319–26.View ArticleGoogle Scholar
- Gabizon A, Isacson R, Libson E, Kaufman B, Uziely B, Catane R, et al. Clinical studies of liposome-encapsulated doxorubicin. Acta Oncol. 1994;33:779–86.View ArticlePubMedGoogle Scholar
- Vermylen J, Nemmar A, Nemery B, Hoylaerts MF. Ambient air pollution and acute myocardial infarction. J Thromb Haemost. 2005;3:1955–61.View ArticlePubMedGoogle Scholar
- Weise G, Basse-Lusebrink TC, Kleinschnitz C, Kampf T, Jakob PM, Stoll G. In vivo imaging of stepwise vessel occlusion in cerebral photothrombosis of mice by 19 F MRI. PLoS One. 2011;6:e28143.View ArticlePubMedPubMed CentralGoogle Scholar
- Nemmar A, Hoylaerts MF, Hoet PHM, Dinsdale D, Smith T, Xu H, et al. Ultrafine particles affect experimental thrombosis in an in vivo hamster model. Am J Respir Crit Care Med. 2002;166:998–1004.View ArticlePubMedGoogle Scholar
- Silva VM, Corson N, Elder A, Oberdorster G. The rat ear vein model for investigating in vivo thrombogenicity of ultrafine particles (UFP). Toxicol Sci. 2005;85:983–9.View ArticlePubMedGoogle Scholar
- Solomon A, Smyth E, Mitha N, Pitchford S, Vydyanath A, Luther PK, et al. Induction of platelet aggregation after a direct physical interaction with diesel exhaust particles. J Thromb Haemost. 2013;11:325–34.View ArticlePubMedGoogle Scholar
- Thogersen AM, Jansson JH, Boman K, Nilsson TK, Weinehall L, Huhtasaari F, et al. High plasminogen activator inhibitor and tissue plasminogen activator levels in plasma precede a first acute myocardial infarction in both men and women: evidence for the fibrinolytic system as an independent primary risk factor. Circulation. 1998;98:2241–7.View ArticlePubMedGoogle Scholar
- Cesari M, Pahor M, Incalzi RA. Plasminogen activator inhibitor-1 (PAI-1): a key factor linking fibrinolysis and age-related subclinical and clinical conditions. Cardiovasc Ther. 2010;28:e72–91.View ArticlePubMedPubMed CentralGoogle Scholar
- Nemmar A, Subramaniyan D, Ali BH. Protective effect of curcumin on pulmonary and cardiovascular effects induced by repeated exposure to diesel exhaust particles in mice. PLoS One. 2012;7:e39554.View ArticlePubMedPubMed CentralGoogle Scholar
- Jacobin-Valat MJ, Laroche-Traineau J, Lariviere M, Mornet S, Sanchez S, Biran M, et al. Nanoparticles functionalised with an anti-platelet human antibody for in vivo detection of atherosclerotic plaque by magnetic resonance imaging. Nanomedicine. 2015;11:927–37.PubMedGoogle Scholar
- Radomski A, Jurasz P, Alonso-Escolano D, Drews M, Morandi M, Malinski T, et al. Nanoparticle-induced platelet aggregation and vascular thrombosis. Br J Pharmacol. 2005;146:882–93.View ArticlePubMedPubMed CentralGoogle Scholar
- Corbalan JJ, Medina C, Jacoby A, Malinski T, Radomski MW. Amorphous silica nanoparticles aggregate human platelets: potential implications for vascular homeostasis. Int J Nanomedicine. 2012;7:631–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Ali LM, Gutierrez M, Cornudella R, Moreno JA, Pinol R, Gabilondo L, et al. Hemostasis disorders caused by polymer coated iron oxide nanoparticles. J Biomed Nanotechnol. 2013;9:1272–85.View ArticlePubMedGoogle Scholar
- Ojha S, Bhatia J, Arora S, Golechha M, Kumari S, Arya DS. Cardioprotective effects of commiphora Mukul against isoprenaline-induced cardiotoxicity: a biochemical and histopathological evaluation. J Environ Biol. 2011;32:731–8.PubMedGoogle Scholar
- Maritim AC, Sanders RA, Watkins III JB. Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Toxicol. 2003;17:24–38.View ArticlePubMedGoogle Scholar
- Li JJ, Muralikrishnan S, Ng CT, Yung LY, Bay BH. Nanoparticle-induced pulmonary toxicity. Exp Biol Med (Maywood). 2010;235:1025–33.View ArticleGoogle Scholar
- Wu X, Tan Y, Mao H, Zhang M. Toxic effects of iron oxide nanoparticles on human umbilical vein endothelial cells. Int J Nanomedicine. 2010;5:385–99.View ArticlePubMedPubMed CentralGoogle Scholar
- Cochran DB, Wattamwar PP, Wydra R, Hilt JZ, Anderson KW, Eitel RE, et al. Suppressing iron oxide nanoparticle toxicity by vascular targeted antioxidant polymer nanoparticles. Biomaterials. 2013;34:9615–22.View ArticlePubMedGoogle Scholar
- Nemmar A, Beegam S, Yuvaraju P, Yasin J, Shahin A, Ali BH. Interaction of amorphous silica nanoparticles with erythrocytes in vitro: role of oxidative stress. Cell Physiol Biochem. 2014;34:255–65.View ArticlePubMedGoogle Scholar
- Alarifi S, Ali D, Alkahtani S, Alhader MS. Iron oxide nanoparticles induce oxidative stress, DNA damage, and caspase activation in the human breast cancer cell line. Biol Trace Elem Res. 2014;159:416–24.View ArticlePubMedGoogle Scholar
- Singh SP, Rahman MF, Murty US, Mahboob M, Grover P. Comparative study of genotoxicity and tissue distribution of nano and micron sized iron oxide in rats after acute oral treatment. Toxicol Appl Pharmacol. 2013;266:56–66.View ArticlePubMedGoogle Scholar