Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons
- Karina Lindner1,
- Michael Ströbele2,
- Sandra Schlick3,
- Sina Webering3,
- André Jenckel4,
- Johannes Kopf5,
- Olga Danov5,
- Katherina Sewald5,
- Christian Buj6,
- Otto Creutzenberg5,
- Thomas Tillmann5,
- Gerhard Pohlmann5,
- Heinrich Ernst5,
- Christina Ziemann5,
- Gereon Hüttmann6,
- Holger Heine4,
- Henning Bockhorn2,
- Tanja Hansen†5,
- Peter König†1Email authorView ORCID ID profile and
- Heinz Fehrenbach†3
© The Author(s). 2017
Received: 15 November 2016
Accepted: 7 March 2017
Published: 21 March 2017
Carbon black nanoparticles (CBNP) are mainly composed of carbon, with a small amount of other elements (including hydrogen and oxygen). The toxicity of CBNP has been attributed to their large surface area, and through adsorbing intrinsically toxic substances, such as polycyclic aromatic hydrocarbons (PAH). It is not clear whether a PAH surface coating changes the toxicological properties of CBNP by influencing their physicochemical properties, through the specific toxicity of the surface-bound PAH, or by a combination of both.
Printex®90 (P90) was used as CBNP; the comparators were P90 coated with either benzo[a]pyrene (BaP) or 9-nitroanthracene (9NA), and soot from acetylene combustion that bears various PAHs on the surface (AS-PAH). Oxidative stress and IL-8/KC mRNA expression were determined in A549 and bronchial epithelial cells (16HBE14o-, Calu-3), mouse intrapulmonary airways and tracheal epithelial cells. Overall toxicity was tested in a rat inhalation study according to Organization for Economic Co-operation and Development (OECD) criteria. Effects on cytochrome monooxygenase (Cyp) mRNA expression, cell viability and mucociliary clearance were determined in acute exposure models using explanted murine trachea.
All particles had similar primary particle size, shape, hydrodynamic diameter and ζ-potential. All PAH-containing particles had a comparable specific surface area that was approximately one third that of P90. AS-PAH contained a mixture of PAH with expected higher toxicity than BaP or 9NA. PAH-coating reduced some effects of P90 such as IL-8 mRNA expression and oxidative stress in A549 cells, granulocyte influx in the in vivo OECD experiment, and agglomeration of P90 and mucus release in the murine trachea ex vivo. Furthermore, P90-BaP decreased particle transport speed compared to P90 at 10 μg/ml. In contrast, PAH-coating induced IL-8 mRNA expression in bronchial epithelial cell lines, and Cyp mRNA expression and apoptosis in tracheal epithelial cells. In line with the higher toxicity compared to P90-BaP and P90-9NA, AS-PAH had the strongest biological effects both ex vivo and in vivo.
Our results demonstrate that the biological effect of CBNP is determined by a combination of specific surface area and surface-bound PAH, and varies in different target cells.
The toxicity of nanoparticles is thought to be determined by their surface reactivity, influenced in turn by their chemical composition, physical properties and surface chemistry [1–8]. Carbon black nanoparticles (CBNP) are composed mainly of carbon with a small amount of other elements (including hydrogen and oxygen), and in themselves are nontoxic. However, CBNP are potentially harmful since their large surface area can interact with biological systems [1, 9–14]. Furthermore, substances such as polycyclic aromatic hydrocarbons (PAHs) that are intrinsically toxic can be adsorbed during CBNP synthesis . The theory that the toxicity of CBNP can be increased by other substances has been validated by the application of metal ions, which increased the toxicity due to autophagy and lysosomal dysfunction . However, it is still not clear how direct binding of chemical add-ons to CBNP modifies their interaction with biological systems [5, 17–19]. Several outcomes are theoretically possible. First, binding of chemicals to the particle surface can modify the surface properties of CBNP, reducing the surface area, thereby potentially reducing toxicological effects . Second, the toxicity of the bound substances can determine the overall toxicity of the resulting particles . Third, the modification could lead to a new particle type, whose biological activity might be a combination of changed physicochemical properties and the toxicity of the bound substances.
A common surface modification of CBNP is the adsorption of PAHs, occurring during incomplete combustion . PAHs are thought to be mutagenic and carcinogenic, generally after toxification by cytochrome P450 enzymes (Cyp), and therefore are regarded as lung tumor inducing substances, although with only very limited acute toxicity [15, 22, 23]. Despite this limited acute toxicity, their effect on biological systems can be easily monitored by their potential to induce specific Cyp enzymes.
Compared with unmodified CBNP, surface modification of CBNP with PAHs has not shown increased tumor induction either following long-term exposure  or in cell lines in terms of cytokine release, induction of single and double strand breaks, cell cycle arrest, or phosphorylation of p53 or histone [25–27]. This suggests that PAHs have limited toxic effects when bound to CBNP, supporting the theory that they are not biologically available in this state  and that surface modification has a minor effect on the overall toxicity of CBNP.
However, diesel exhaust particles that contain PAHs are associated with an increased risk of asthma, and experiments involving exposure of a bronchial cell line to ultrafine particles implied a role of PAH in their toxic effects [29–31]. Furthermore, removal of PAHs from these exhaust particles resulted in reduced carcinogenic potential . These data suggests that the effect of PAHs on the behavior of CBNP in biological systems is more complex.
Since inhalation is the predominant route of exposure to CBNP, we tested the toxicity of particles in different test systems including cell cultures, ex vivo studies on excised airways, and an in vivo inhalation study conducted according to Organization for Economic Co-operation and Development (OECD) criteria . Initially, we tested the CBNP in high-throughput cell culture systems that can predict the toxicity of particles, and which therefore allowed us to preselect CBNP for in vivo testing. We then analyzed the in vivo effects of the selected CBNP in inhalation studies, and then finally tested the CBNP in ex vivo airway preparations to determine their impact on ciliary beat frequency, particle transport speed and mucus appearance. In every test system we analyzed parameters that indicate CBNP-induced oxidative stress and IL-8 response.
This allowed us to compare specific toxicological effects in each system to gain a more comprehensive insight into the cellular effects of the particles.
To understand how surface modification can change the interaction of CBNP with biological systems we used PAHs to modify the surface of a toxicologically well-defined CBNP, Printex®90 (P90). This is characterized by a high surface area and has been widely used in toxicological studies, resulting in only minor toxic effects [1, 33, 34].
For modification of the P90 surface, we used benzo[a]pyrene (BaP) and 9-nitroanthracene (9NA). BaP was chosen because of the well characterized toxicity of its metabolites, which are known to induce ROS and DNA adducts [23, 35–38]. BaP is known to induce Cyp1A1 and 1B1, which then metabolize BaP to toxic metabolites; this therefore allows monitoring of BaP activity and its biological effect [39, 40]. In contrast, 9NA is a PAH that occurs during combustion, and is regarded as a low toxicity PAH, as predicted by the Ames test and human cell mutagenicity assay [41–43]. However, due to its nitro group other toxic mechanisms can occur induced by intermediates resulting from reduction of the nitro group . As coating of a particle does not necessarily represent the situation found in nanoparticles that acquire PAH during synthesis, we also generated CBNP by acetylene combustion . The resulting acetylene soot (AS) had a mixture of PAHs on the surface (AS-PAH). In the suspensions we used, AS-PAH had a slightly larger specific surface area, but similar aggregate size and ζ-potential compared to PAH-coated P90, The physicochemical parameters of the different particles were evaluated by a variety of analytic test methods (see Tables 1, 2 in the Results section and Additional files 1, 2).
Particle synthesis and characterization
Modification of CBNP
80 g of unmodified P90 (Evonik Carbon Black GmbH, Frankfurt, Germany) together with 20 g of BaP (Thermo Fisher Scientific-Alfa Aesar, Heysham, Lancashire, UK) or 9NA (Merck Schuchardt OHG, Hohenbrunn, Germany) were suspended in 100 ml dimethyl ether and incubated in an ultrasonic bath at room temperature for 15 min. After subsequent vacuum desiccation, the coated P90 nanoparticles were stored at room temperature.
Gas phase synthesis of AS-PAH
A flat flame burner in a low pressure combustion chamber with acetylene as the fuel was used for the synthesis of AS-PAH. The experiments were carried out with a carbon to oxygen ratio of 1.1 to 1. The laminar mass flow of acetylene was set via mass flow controllers (Brooks 5850E; Brooks Instruments, Hatfield, PA, USA) on 88 ln/h with the oxygen mass flow adjusted to 80 ln/h. Temperature and pressure were monitored by a thermocouple and a pressure sensor, with the pressure set to 70 mbar during synthesis. The AS-PAH was collected for 60 min on a filter (Gore Membrane Filter Bags) between the combustion chamber and the rotary vane pump (TRIVAC D 65 B; Oerlikon Leybold Vakuum, Cologne, Germany).
Particle size distribution
The particle size distributions were measured by scanning electron microscopy (SEM; Leo 1530; LEO Electron Microscopy Inc., Thornwood, NY, USA) and transmission electron microscopy (TEM; CM200 FEG/ST, Philips, Amsterdam, Netherlands). The PAHs on the particle surface had to be extracted with toluene prior to electron microscopy, as otherwise they would be cracked by the beam and the particles would grow. Remaining CBNP were then suspended with acetone in an ultrasonic bath, and the suspension was applied thinly using an ultrasonic nebulizer, either to a silicon wafer (for SEM) or to a 400 mesh cooper grid (Ted Pella, Inc., Redding, CA, USA) (for TEM). The diameter of 200 particles of 5 images was determined with the program ImageJ. A log normal curve was fitted on the particle size distribution to get the average particle diameter.
The thermal release of coated PAHs from P90 and of volatile substances from AS-PAH was investigated using a thermogravimetric analyzer (DuPont 951; DuPont Company, Wilmington, DE, USA) connected to a quadrupole mass spectrometer (QMG 420; Balzers AG, Balzers, Liechtenstein) through a differentially pumped inlet system. The transfer line between the thermobalance and the mass spectrometer was heated up to 400 °C to prevent condensation of the pyrolysis products. The experiments were performed with sample sizes of 5–10 mg at a heating rate of 10 °C/min up to a final temperature of 800 °C at a helium flow rate of 100 ml/min. Each sample was measured at least three times.
Identification of PAHs on AS-PAH surface
To characterize the PAHs on the particle surfaces, CBNP samples were heated under dynamic conditions. A sample of 100 mg was heated up in an oven in 10 °C/min increments to vaporize the PAHs, with the volatile PAHs collected in a cold trap. The condensed products were dissolved in 5 ml toluene, and were characterized by a standard gas chromatograph-mass spectrometer (GC/MS-QP 2010SE; Shimadzu Corporation, Tokyo, Japan). The chromatographic parameters for GC/MS were: Phenomenex zb-5msi column (column length: 30 m; I.D.: 0.25 mm; film thickness 0.25 mm); injector temperature 250 °C; oven temperature program: 65 °C at 5 min constant, 10 °C min to 350 °C at 10 min constant.
Specific surface area
The surface area was determined by Brunauer, Emmett and Teller (BET) measurement using a BelSorp II device (BelJapan, Inc., Osaka, Japan), using the BelSorp analysis program.
Particle size and ζ-potential in suspension
Suspensions were prepared with 10 mg of sample plus 100 ml of double-deionized water. To keep the suspension stable, 500 mg of bovine serum albumin (BSA) (AppliChemInc., Maryland Heights, MO, USA) was added. The suspensions were treated with an ultrasonic homogenizer (Sonoplus, Rod: VS 70 D; Bandelin electronic GmbH & Co. KG, Berlin, Germany) for 40 min in pulse mode, changing 20 times between 30 s ultrasonic and 90 s resting interval. To avoid thermal denaturation of BSA, the suspensions were cooled in a beaker containing ice water during treatment. The particle size measurements were carried out as soon as the suspension had reached room temperature using a Malvern ZetaSizer Nano-ZS (Malvern Instruments Ltd, Worcestershire, UK). Particle size was quantified by dynamic light scattering, and ζ-potential was measured by laser Doppler microelectrophoresis using the Smoluchowski model. The index of refraction and the absorption were preset at 2.0 in the measurement software (DTS Nano; Malvern Instruments Ltd, Worcestershire, UK). The cuvettes to measure the particle size distribution and the ζ-potential were washed using double-deionized water and ethanol (70%) to remove potential impurities. Subsequently, the cuvettes were rinsed using approximately 2 ml of the sample, and were then filled with the sample and placed in the Zetasizer Nano-ZS. The suspensions were characterized in triplicates, and the means and standard derivations were calculated.
CBNP suspensions were tested for endotoxins using the CROMO-LAL assay (Associates of Cape Cod Inc., East Falmouth, MA, USA) according to the manufacturer’s protocol. The limit of detection was 0.005 EU/ml (comparable to 0.5 pg/ml endotoxin of E. coli Type 055:B5). The suspensions were only used for experiments if no endotoxins were detected.
Experiments with human pulmonary cell lines
We used A549 cells (DSMZ, Braunschweig, Germany), 16HBE14o- cells (University California, San Francisco, USA; described in ) and Calu-3 cells (American Type Culture Collection, Manassas, VA, USA). The epithelial cells were cultured in Dulbecco’s modified eagle’s medium (DMEM) with 10% fetal calf serum (FCS) and 0.1% gentamicin for ROS and transepithelial electrical resistance (TEER) measurements. For mRNA expression analysis, A549 cells were cultured in DMEM (Fisher Scientific GmbH, Schwerte, Germany) with 10% FCS and 0.1% gentamicin, 16HBE14o- cells were cultured in DMEM (Biochrom AG, Berlin, Germany) with 10% FCS (PAA Laboratories GmbH, Cölbe, Germany) 1% L-glutamine (Gibco™, Life Technologies GmbH, Darmstadt, Germany) and 1% penicillin/streptomycin (Sigma Aldrich Chemie GmbH, Taufkirchen, Germany), and Calu-3 cells were cultured in RPMI 1640 containing stable glutamine supplemented with 10% FCS, 100 U/ml penicillin and 100 μg/ml streptomycin (all from Biochrom AG, Berlin, Germany). Cells were kept in a 37 °C humidified atmosphere containing 5% CO2.
Flow cytometric measurements of reactive oxygen species (ROS)
Cells were seeded in T25 flasks (PAA Laboratories GmbH, Cölbe, Germany), at 0.6 × 106 for A549 cells and at 1 × 106 for 16HBE14o- cells. Subconfluent cultures were exposed to CBNP at a concentration of 10 μg/ml or 50 μg/ml for 24 h. The culture medium was subsequently removed and the cell monolayers were washed twice with 5 ml pre-warmed phosphate buffered saline (PBS). The fluorescent dye dichloro-dihydro-fluorescein diacetate (DCFH-DA) was applied to the cells in 5 ml fresh medium, with the resulting mixture incubated for 30 min at 37 °C. The culture medium was then removed and the cells were washed twice with 5 ml pre-warmed PBS and harvested by trypsinization. Flow cytometry measurements were done with a FACScan cytometer (Becton Dickinson Biosciences, San Jose, CA, USA) equipped with a 488 nm argon laser, with CELLQuest™ software used for data acquisition. For each sample, 10,000 events were analyzed and fluorescence emission of dichlorofluorescein (DCF) was examined at 530 nm.
A549 cells were seeded in T25 flasks at 0.6 × 106. Calu-3 cells and 16HBE14o- cells were seeded in 12-well plates (Costar, Corning, NY, USA) at 5 × 105 cells and 1.6 × 105 cells, respectively. After reaching a confluency of ≥90%, culture medium was exchanged and cells were treated with CBNP at 10 μg/ml, 50 μg/ml or medium/BSA alone for 24 h. Subsequently, cells were washed twice with pre-warmed medium or PBS to remove particles, and total RNA was extracted from Calu-3 cells using a NucleoSpin RNA Kit (Macherey-Nagel, Düren, Germany) and from A549 and 16HBE14o- cells using a RNeasy Kit (Quiagen GmbH, Hilden, Germany) according to the manufacturers’ instructions.
Reverse transcription was performed for RNA of A549 cells with the Omniscript Kit (Quiagen GmbH, Hilden, Germany), of 16HBE14o- cells using MaximaFirstStrand cDNA synthesis (Fermentas/Life Technologies GmbH, Darmstadt, Germany), and of Calu-3 cells using Oligo(dT)12–18 and Superscript III reverse transcriptase (Thermo Fisher Scientific, Waltham, USA), all according to the manufacturers’ protocols.
To quantify IL-8 and hypoxanthine-guanine phosphoribosyltransferase (HPRT) mRNA levels in A549 cell preparations, we used Viia™7 (Life Technologies GmbH, Darmstadt, Germany); for preparations of 16HBE14o- and Calu-3 cells, we used LightCycler 480 SYBR green I Master (Roche Applied Science, Mannheim, Germany).
The sequences of used IL-8 primers and housekeeping gene HRPT1 primers are shown in Additional file 3. The housekeeping gene was evaluated with GenEx software (MultiD Analyses AB, Gothenburg, Sweden) . The quality of amplificates was tested by melting curve analysis. The n-fold change was calculated for A549 cells with the program GenEx (GenEx Professional 5.4.3 Software), and by normalization of relative expression data to medium control data for 16HBE14o- and Calu-3 cells.
Measurement of the transepithelial electrical resistance (TEER)
A total of 2 × 105 Calu-3 cells were seeded per well on Transwell® filter inserts (polyester, 12-well plates, 12 mm diameter, 0.4 μm pore size; Corning Costar, Bodenheim, Germany) and cultivated for 9 days. On day 10, the cell monolayers were exposed to CBNP at 10 μg/ml or 50 μg/ml for 24 h. The culture medium was changed and the cells were incubated for at least 15 min at 37 °C, before TEER was measured using an EVOM volt-ohm-meter (World Precision Instruments, Sarasota, Fl, USA).
Differences between untreated controls and treated cultures were considered statistically significant at p < 0.05. Flow cytometric and TEER data were analyzed by two-sided Student’s t-test for unpaired values using Microsoft Excel. Relative mRNA expression results were analyzed by Mann Whitney U test.
Nose-only inhalation study in rats
Male Wistar rats (strain Crl:WI (Han)) were purchased from Charles River Deutschland (Sulzfeld, Germany). The age of the animals was 10 to 11 weeks and the weight 285 ± 24 g (mean ± SD) at the onset of exposure. Rats were exposed to the test item by nose-only inhalation. For 3 weeks prior to exposure, animals were trained to become accustomed to nose-only tubes. The OECD inhalation study on rats was approved by the Niedersächsisches Landesamt für Verbraucherschutz und Lebensmittelsicherheit (33.14 42502-04013/1199).
Study design and dosing scheme
The CBNP were administered to the test animals by nose-only inhalation. P90, P90-BaP and AS-PAH were tested; P90-9NA was not administered, given the previously described low toxicity [41–43] and the in vitro tests in this study. A nominal aerosol concentration of 6 mg/m3 was used, with exposure of 6 h/day for two weeks (no exposure on weekends) according to the OECD criteria for acute exposure . CBNP-related effects on innate immune cells in the bronchoalveolar lavage (BAL) and histopathological changes in lung tissue were analyzed on day 1 and day 14 after cessation of exposure. Potassium bromate (KBrO3) treated animals (250 mg/kg in physiological saline, intraperitoneal) served as positive controls for Comet-assay. The animals were treated with KBrO3 on day 1 and day 14. BAL was collected and analyzed 3 h after application.
Bronchoalveolar lavage and leukocyte determination
BAL was performed in five rats per group after the end of exposure (day 1) and following a 14-day recovery period. Following preparation, lungs were lavaged with saline using two lavages of 5 ml each. The lavage fluid was collected in calibrated tubes and the harvested volume was recorded. Leukocyte concentration was determined using a counting chamber, and two cytoslides were prepared with a cytocentrifuge (Shandon Co., Frankfurt, Germany) for differential cell counts (macrophages, granulocytes, lymphocytes). The slides were air dried, and the cells were stained with Diffquick solutions (Medion Grifols Diagnostics AG, Düdingen, Switzerland) and counted. The results are presented as percentage of the total number of BAL cells.
Differences between BAL analysis groups were considered statistically significant at p < 0.05. Data were analyzed using analysis of variance. If the group means differed significantly by the analysis of variance the means of the treated groups were compared with the means of the control groups using Dunnett’s test.
Experiments with explanted mouse trachea
Particle suspension in cell culture medium
For the ex vivo studies we used CBNP concentrations of 10 and 30 μg/ml. The concentration of 30 μg/ml was calculated by surface correction of the in vitro concentration.
The CBNP suspensions were diluted in DMEM (Gibco™, Life Technologies GmbH, Darmstadt, Germany) with 20 mM HEPES, 1% penicillin/streptomycin (Sigma Aldrich Chemie GmbH, Taufkirchen, Germany) and 2 mM L-glutamine (Life Technologies GmbH, Darmstadt, Germany) for incubation of tracheae.
We used 8 to 12 week old female Balb/c mice (Charles River Laboratories, Sulzfeld, Germany). Mice were euthanized with an overdose of isoflurane for explantation of the trachea. The ex vivo studies on mouse tracheae were approved by the Ministerium für Landwirtschaft, Umwelt und ländliche Räume des Landes Schleswig-Holstein.
Preparation and culture conditions of explanted trachea
Tracheae were explanted and transferred to culture dishes coated with a thin layer of Sylgard polymer and filled with medium. Each trachea sample was fixed on the bottom of a well with an insect needle, covered with 1 ml of medium with or without suspended CBNP and incubated at 37 °C for 24 h.
To examine differences in mRNA expression levels, tracheae were cut into two pieces. One piece was incubated for 24 h with particle suspension; the other piece was incubated in medium only, serving as negative control. The epithelium was then removed using a sterile swab (Collection Swab H268A; Copan diagnostic, Inc., Murrieta, CA, USA). This isolation method was previously described in . The RNA was isolated with innuPREP RNA isolation Kit (Analytic Jena AG, Jena, Germany) according to the manufacturer’s protocols. Any remaining DNA was enzymatically degraded using Amplification Grade DNase I kit AMPD-1 (Sigma-Aldrich Chemie GmbH, Steinheim, Germany). The quality and quantity of isolated mRNA was analyzed with a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). The mRNA was subsequently transcribed to cDNA using RevertAid H Minus Reverse Transcriptase (Fisher Scientific-Germany GmbH, Schwerte, Germany) mixed in transcription buffer with dNTPs (0.6 mM) (Fisher Scientific-Germany GmbH, Schwerte, Germany), DTT (10 mM) (Life technologies GmbH, Darmstadt, Germany) and random hexamer primers (17.5 ng/μl) (Biomers.net GmbH, Ulm, Germany).
Real time RT-PCR was performed in TaqMan Universal PCR Master Mix (Life technologies GmbH, Darmstadt, Germany), with probes tagged with reporter 6-FAM and quencher Tamra. The TaqMan probes and primers used for quantitative real time RT-PCR are shown in Additional file 3. The PCR program was: one cycle at 95 °C for 10 min followed by 50 cycles at 95 °C for 45 s and at 60 °C for 1 min. Changes in the transcript levels were determined by calculating the difference in cycle threshold to the respective negative control with the ΔΔCt method and represented as n-fold expression (2 −ΔΔCt).
Apoptosis and necrosis assays
Tracheae were explanted, incubated in pre-warmed medium, opened and cut in four equal parts. One part was cultured in medium alone, two parts were incubated for 24 h with CBNP suspensions of 10 μg/ml and 30 μg/ml, respectively, and the remaining parts were incubated for 24 h with 1 μM staurosporine (Roche Diagnostics GmbH, Mannheim, Germany) or 1 mM paraquat (Sigma-Aldrich GmbH, Seelze, Germany), respectively as positive controls for the staining procedure.
For the apoptosis analysis, whole mount samples of tracheae were fixed with phosphate buffered 4% paraformaldehyde (pH 7.4), followed by washing with PBS (pH 7.4) and permeabilization with ice cold acetone. The specimens were then incubated overnight at room temperature with rabbit anti-cleaved caspase-3 antibody (#9661, dilution 1:300, Cell Signaling Technology Inc., Danvers, MA, USA). The primary antibody was detected using Alexa555-conjugated donkey anti-rabbit IgG (dilution 1:800, Life Technologies GmbH, Darmstadt, Germany).
For membrane damage analysis, ethidium homodimer-1 (1 μg/ml, Life Technologies GmbH, Darmstadt, Germany) in medium was added to the trachea preparation, and was then incubated for 30 min at 37 °C. The samples were fixed with 4% paraformaldehyde (pH 7.4), washed with PBS (pH 7.4) and permeabilized with acetone.
To identify the epithelial layer, ciliated cells were labeled with a mouse monoclonal antibody against acetylated α-tubulin (Clone 6-11B-1, Sigma-Aldrich GmbH, Steinheim, Germany) that was directly labeled with Atto488 (Lightning-Link™Atto488 conjugation kit; Innova Bioscience Ltd., Cambridge, UK); cell nuclei were visualized using Hoechst 33258 (0.1 μg/ml; Sigma-Aldrich, Steinheim, Germany) (Additional file 4).
Apoptosis and cell membrane damage were analyzed using a confocal laser scanning microscope (LSM 510 Meta; Carl Zeiss MicroImaging GmbH, Göttingen, Germany) equipped with a Plan-Apochromat 20×/0.75 or C-Apochromat 40×/1.2 W objective. The total number of apoptotic and necrotic epithelial cells was counted in the complete whole mount preparation.
Ciliary beat frequency and cilia-driven particle transport
Tracheae were transferred to a Delta T4 Culture dish (Bioptechs Inc., Butler, PA, USA), the bottom of which was covered with sylgard polymer. Samples were fixed with insect needles with the epithelium facing upwards, and were then covered with 2 ml of HEPES-Ringer solution. The temperature of HEPES-Ringer solution was maintained at 30 °C using a Bioptechs Delta T4 Culture Dish Controller (Bioptechs Inc., Butler, PA, USA), and the epithelial surface was imaged using a Zeiss Axioskop 2 FS fixed stage microscope equipped with Achroplan 40×/0.80 W or 20×/0.50 W objectives (Carl Zeiss MicroImaging GmbH, Göttingen, Germany).
Polystyrene particles (4.5 μm) were added to the HEPES-Ringer solution with a concentration of 2 × 106 particles/ml. The transport of particles was recorded with a SMX-150 M camera (EHD Imaging GmbH, Damme, Germany). Movies (each with 200 images) of polystyrene particle transport were recorded from at least six different areas for each trachea at 12 Hz frame rate and a resolution of 1280 × 1024 pixels. Particle transport speed was determined by tracking individual particles and manually checking each recorded track using Image-Pro® Plus 6.0 (Medium Cybernetics, Inc., Bethesda, MD, USA). The mean particle transport speed was calculated for each experiment. Movies (each with 1000 images) of cilia motion were recorded at 100 Hz frame rate and 640 × 480 pixels). The ciliary beat frequency was determined by Fourier transformation of grey level changes over time using an in-house developed software written in Mathlab (Mathworks, Natick, MA, USA). For visualization of ciliary beat frequency, the dominant frequency, i.e. the frequency with the highest amplitude, was identified for every pixel in the movie and color-coded. Movies were recorded from at least six different areas for each trachea. The ciliary beat frequency of a minimum of 10 ciliated cells was measured in every movie and the mean frequency from these cells was calculated. To increase ciliary beat frequency or cilia-driven particle transport, ATP (10 μM) was applied as positive control.
All ex vivo experiments were carried out at least three times. Quantitative RT-PCR data are presented as mean ± SEM. The data sets were analyzed using paired-sample Wilcoxon signed-rank test in GraphPad Prism 5 (GraphPad Software, Inc., La Jolla, CA, USA). P values < 0.05 were considered statistically significant.
Mucus staining in tracheal whole mounts
Tracheae were incubated in 1 ml HEPES-Ringer solution at 30 °C; 1 μl of a 1:1 mix of wheat germ agglutinin (WGA) and Ulex europaeus agglutinin (UEA-1) was added (each 1 μg/ml). WGA stains N-acetyl-D-glucosamine and sialic acid residues, whereas UEA-1 has an affinity for L-fucose, all of which are carbohydrate residues present in mucins [48, 49]. In addition, WGA labels ciliated cells . The staining was imaged with an AxioMR camera and an Achroplan 40×/0.8 W objective with a resolution of 1388 × 1040 pixels.
Further information on the determination of cytokine levels and cell viability in vitro, CBNP aerosol generation and exposure of rats, oxidative Comet-Assay, histopathology of the rat lungs, particle suspensions in cell culture medium, preparation and culture conditions of intrapulmonary airways, qualitative RT-PCR, macroscopic analysis, scanning electron microscopy and individual statistics are provided in Additional file 5.
Characterization of CBNP and their suspensions
Characteristics of dry and suspended CBNP
Mean primary particle size (nm)
16.5 ± 0.4a
16.5 ± 0.4a
16.5 ± 0.4a
14.2 ± 0.1a
Mass loss (%)
0.5 ± 0.4
16.1 ± 0.5
14.6 ± 0.1
19.0 ± 0.7
Specific surface area (m2/g)
302 ± 16
91 ± 2
115 ± 3
Hydrodynamic diameter in water/BSA (nm)
155 ± 1
176 ± 5
172 ± 7
174 ± 5
ζ-potential in water/BSA (mV)
−34 ± 5
−38 ± 4
−37 ± 3
−36 ± 3
Hydrodynamic diameter in medium (nm)
163 ± 6
160 ± 8
156 ± 11
170 ± 4
ζ-potential in medium (mV)
−15 ± 1
−13 ± 1
−13 ± 1
−12 ± 1
AS-PAH exhibited a slightly larger specific surface area and mass loss compared with the coated P90 nanoparticles, and AS-PAH without PAHs showed a slightly smaller mean primary particle size than uncoated P90 (Table 1 and Additional file 1).
The thermogravimetric (TG) analysis of AS-PAH indicated a two-stage thermal desorption of surface components (Additional file 2B). The first stage ended at approximately 240 °C and mainly included PAHs (Additional file 2C). Because of possible defragmentation, the exact PAH content cannot be determined, but a minimal PAH content of approximately 11% was calculated via TG curve correlating with the mass loss at 240 °C (Additional file 2C). In the second stage, hydrogen, methane, water, acetylene and carbon dioxide were mainly released (Additional file 2D).
Identified PAHs extracted from the AS-PAH surface
Retention time (min)
IARC carcinogens classification
Analysis of CBNP effects in vitro
The in vitro effects of CBNP depended on the cell line
In vitro results of ROS, IL-8 mRNA expression and TEER measurement after exposure to CBNP
Relative DCF fluorescence
Relative DCF fluorescence
1.2 ± 0.2
1.2 ± 0.4
1.5 ± 0.2**
1.4 ± 0.2
103 ± 7
1.0 ± 0.2
2.1 ± 0.5*
2.1 ± 0.8**
3.2 ± 1.1**
1.7 ± 0.9
109 ± 8
1.1 ± 0.0
1.1 ± 0.1
0.7 ± 0.2
1.3 ± 0.1
0.7 ± 0.3
97 ± 8
1.9 ± 0.6**
1.2 ± 0.1
0.7 ± 0.2
2.1 ± 0.1**
1.9 ± 1.8
94 ± 9
3.0 ± 0.9**
0.9 ± 0.1
0.8 ± 0.2
1.1 ± 0.1
1.1 ± 0.5
105 ± 10
1.1 ± 0.1
1.2 ± 0.2
0.8 ± 0.2
2.1 ± 0.3**
2.0 ± 0.9*
104 ± 9
1.2 ± 0.1
2.3 ± 0.2**
0.8 ± 0.3
1.8 ± 0.2**
1.1 ± 0.4
91 ± 7*
1.1 ± 0.2
3.0 ± 0.4**
0.8 ± 0.3
1.9 ± 0.1**
1.4 ± 0.8
90 ± 6*
2.1 ± 1.2**
16HBE14o- cells are regarded as model for bronchial epithelial cells. In these cells, all CBNP induced ROS formation at 50 μg/ml, but only P90-9NA increased IL-8 mRNA expression. Calu-3 cells are models for both bronchial epithelial cells and epithelial barrier function. In these cells, P90-BaP and AS-PAH induced IL-8 mRNA expression; only AS-PAH reduced TEER (Table 3). AS-PAH-induced IL-8 protein release was significantly increased compared to that following P90 exposure (Additional file 6A). IL-6 protein was significantly increased in the supernatant after exposure to 50 μg/ml P90-BaP, and following exposure to both concentrations of AS-PAH (Additional file 6B). Cell viability was not significantly affected by the tested CBNP at either concentration (Additional file 6C).
In vivo nose-only inhalation study in rats
CBNP aerosol characterization
Aerosol concentrations and mass median aerodynamic diameters (MMADs) are summarized in Additional file 7. The mean concentrations were close to the target concentration (6 mg/m3) for all aerosols, and all MMADs were below 3 μm.
Only AS-PAH increased the relative lung weights and induced a moderate inflammatory response
On day 1 post-exposure, there was a statistically significant increase in polymorphonuclear granulocyte numbers in the animals exposed to P90 and AS-PAH compared to the clean air control group (Fig. 1). In AS-PAH treated animals, numbers of polymorphonuclear granulocytes were still moderately increased on day 14 compared to clean air treated animals, indicating the presence of a mild chronic inflammatory response (Fig. 1). None of the tested CBNP influenced LDH, β-glucuronidase activity, or total protein levels in BAL fluid (Additional file 9A), and none induced oxidative DNA damage in BAL cells (Additional file 9B).
CBNP exposure induced very slight histological alterations (Additional file 10). However, only AS-PAH induced bronchiolo-alveolar hyperplasia, observed in two of five animals on day 14 post-exposure (Additional file 10).
Ex vivo airway models
As the in vivo experiments did not specifically address airway epithelial function but in vitro experiments indicated that airway epithelial cells are sensitive to CBNP effects, we examined the particle effects on explanted murine airways.
Only AS-PAH caused a pro-inflammatory response in the tracheal epithelium
Results of mRNA expression in tracheal epithelial cells after CBNP exposure
1.1 ± 0.2
1.1 ± 0.2
1.2 ± 0.2
0.7 ± 0.2
1.3 ± 0.2
1.2 ± 0.3
3.8 ± 1.5*
2.4 ± 0.6*
0.7 ± 0.2
0.8 ± 0.1
1.0 ± 0.2
0.6 ± 0.1*
1.3 ± 0.3
0.7 ± 0.1*
2.0 ± 0.8
2.6 ± 0.4*
2.2 ± 0.9
0.7 ± 0.1*
0.8 ± 0.2
0.8 ± 0.1
1.7 ± 0.7
0.7 ± 0.1
5.2 ± 3.8
1.3 ± 0.5
0.7 ± 0.1*
0.7 ± 0.1*
1.0 ± 0.1
1.0 ± 0.1
1.4 ± 0.2
1.0 ± 0.2
1.5 ± 0.3
1.7 ± 0.5
1.4 ± 0.4
0.8 ± 0.3
331 ± 22*
1289 ± 412*
2.0 ± 1.0
30 ± 9*
1157 ± 232*
2442 ± 452*
0.2 ± 0.1*
0.2 ± 0.0*
5.3 ± 1.0*
10.9 ± 2.4*
1.3 ± 0.1
2.5 ± 0.6*
11.1 ± 4.3*
4.3 ± 0.7*
All PAH containing CBNP induced Cyp-expression but none of the CBNP induced mRNA for indicators of oxidative stress in tracheal epithelial cells
We analyzed the mRNA expression of the cytochrome P450 enzymes Cyp1A1 and Cyp1B1 as indicators of PAH metabolism, and of Gpx3 and Gr as indicators of oxidative stress.
All PAH-containing CBNP induced Cyp1a1 and Cyp1b1 mRNA in the tracheal epithelium (Table 4). The most potent CBNP was AS-PAH, followed by P90-BaP. P90-9NA only induced Cyp1a1 and Cyp1b1 mRNA expression at 30 μg/ml. In contrast, the uncoated P90 nanoparticles did not induce Cyp1a1 mRNA expression, and significantly decreased Cyp1b1 mRNA expression compared to medium controls in tracheal epithelial cells (Table 4). None of the CBNP increased the mRNA expression of Gpx3 or Gr. In intrapulmonary airways PAH-containing CBNP induced a similar expression pattern of Cyp1a1 mRNA compared to the tracheal epithelium; no increase of mRNAs for anti-oxidative enzymes was detected (Additional file 11).
Since the mRNA data of Cyp-expression indicated that the CBNP affect airway epithelial cells and to detect changes in epithelial function, we assessed their impact on cilia-driven transport.
Only P90 forms microscopically visible agglomerates; these bind to mucus and interact with ciliated cells
Additional file 13: P90 agglomerates attached to cilia of ciliated cells after incubation with 10 μg/mlP90. The movie shows cilia movement and attached P90 agglomerates on cilia of tracheal epithelium after 24 h exposure to 10 μg/ml P90. The movie was recorded with 100 Hz and a resolution of 640 × 480 pixels. The image sequence contains 1000 images. (WMV 11853 kb)
Additional file 14: P90 agglomerates attached to cilia of ciliated cells after incubation with 30 μg/ml P90. The movie shows the cilia movement and bound P90 agglomerates on the cilia of tracheal epithelium after 24 h exposure to 30 μg/ml P90. The movie was recorded with 100 Hz, a resolution of 640 × 480 pixels and contains 1000 images. (WMV 11658 kb)
At 10 μg/ml, P90 agglomerates increased ciliary beat frequency but mucus reduced particle transport speed
Compared to medium control, P90 at 10 μg/ml increased mean ciliary beat frequency (Fig. 2c), although it did not increase the mean of cilia-driven particle transport speed (Fig. 2d). Cells with bound agglomerates exhibited a higher ciliary beat frequency compared to cells without visible agglomerates (Additional file 12B and C). Furthermore, particle transport speed was reduced in areas with mucus and increased in areas without mucus compared to medium control (Additional file 12D). Incubation with P90 at a concentration of 1 μg/ml did not result in formation of agglomerates, and therefore did not increase ciliary beat frequency (Additional files 12A and 15A). However, this concentration was sufficient to increase mucus (Additional files 15B and C), and consequently to reduce particle transport speed (Additional file 15D).
At 10 μg/ml, PAH-coated P90 did not increase ciliary beat frequency but differently influenced mucus release and particle transport speed
As expected from the inability to form agglomerates, neither P90-BaP nor P90-9NA increased ciliary beat frequency (Fig. 2e and f). Incubation with P90-BaP increased amounts of mucus on the epithelial cells (Additional files 16 and 17), with a consequent reduced particle transport speed compared to control (Fig. 2g and h). In contrast to P90 at 10 μg/ml, we observed dead cells on the epithelium during the experiments with both coated particles (Additional files 16, 17 and 18).
At 10 μg/ml, AS-PAH increased ciliary beat frequency and mucus release but reduced particle transport speed
Although AS-PAH did not form agglomerates, it increased ciliary beat frequency and resulted in mucus release (Fig. 2i, and Additional files 12A and 19). Compared to control, AS-PAH reduced particle transport speed (Fig. 2j), and, as with both coated particles, we observed dead cells on the epithelium (Additional file 19).
At 30 μg/ml, all particles resulted in reduced particle transport speed
At 30 μg/ml, none of the particles increased ciliary beat frequency but all reduced particle transport speed (Fig. 2k to n and Additional file 20). All particles increased mucus, and dead cells were visible on the epithelium (Additional files 16, 17, 18 and 19).
PAH-containing particles induced apoptosis in tracheal epithelial cells
Our results suggest that the biological effect of PAH-coating is a combination of specific surface area and PAH-specific effects.
In our study, we used four CBNP: P90, P90 coated with BaP (P90-BaP), P90 coated with 9NA (P90-9NA), and, to model nanoparticles that acquire PAH during synthesis, a soot derived from acetylene combustion that contains various PAHs (AS-PAH). This allowed us to examine how PAH coating changes the behavior of the P90 nanoparticle in various test systems. These particles were tested in vitro, ex vivo and in vivo.
Nanoparticle reactivity is determined by the surface reactivity, including the nanoparticle characteristics: size, shape, solubility, surface area, and chemical composition [2, 5, 8, 9, 51, 52]. All particles that we used had similar primary particle size, shape, and hydrodynamic diameters and ζ-potential in medium. An equal solubility was accomplished by adding protein, resulting in negative ζ-potential at physiological pH-value. Thus, any differences observed are attributable to the surface area or surface chemical composition of the CBNP. Coating of P90 with either PAH reduced its surface area from 302 m2/g to 91 m2/g; since the effects of P90 in biological systems are attributed to its high surface area, this reduction would be expected to reduce the observed biological effects [1, 10].
BaP is considered a tumorigenic reference PAH, with well characterized effects, including induction of ROS formation, IL-8 release and apoptosis [6, 15, 53–57]. It is metabolized by Cyp1A1 and 1B1, and has been shown to upregulate these enzymes both on mRNA and protein levels [23, 58]. Thus, increases in Cyp1a1 and 1b1 mRNA indicate that this PAH is bioavailable in cells. PAH with 4 and more aromatic rings and specific structural features (Bay- or Fjord-region) are strong Cyp inducers and considered to be tumorigenic [15, 59]. Since 9NA is composed of only three aromatic rings, it is less toxic than BaP and considered as weak Cyp inducer [15, 43] as confirmed by our Cyp1a1 and Cyp1b1 mRNA expression results.
Since it is possible that coating of CBNP may result in a different distribution of PAH compared to particles that acquire PAH during synthesis, we also used AS-PAH synthesized from acetylene combustion as a test compound [44, 60]. Since AS-PAH has a similar surface area, shape, size and solubility compared to PAH-coated P90, the different effects of these particles observed in the biological systems are likely the result of its different PAH composition [61, 62]. The AS-PAH that we synthesized contained a mixture of at least 17 PAH with 2–7 aromatic rings that could be removed from the particle with organic solvents, indicating that these PAH are on the surface of the particle. Several of the identified PAH are predicted to be metabolized by Cyp1A1 and 1B1 and induce their mRNA. Thus, by determining the mRNA expression of both enzymes allowed us to determine if the PAHs from AS-PAH are bioavailable. The presence of PAHs with 5 or more aromatic rings predict that, compared to BaP, the PAH mixture of AS-PAH would be more toxic [15, 63]. For example, compared with BaP the 5-ring component cyclopenta[cd]pyrene induced the same mutation mechanism , a stronger lung tumorigenic response in A/J mice  and was 6.9 ± 4.2-fold more mutagenic in vitro . In addition, PAH-mixtures are often more toxic than would be expected by simple addition of the individual PAH toxicities [65, 66]. Thus, when not bound to CBNP the PAH mixture from AS-PAH is likely to be more toxic compared to BaP. Since the relative in vivo effects of PAHs depend on their bioavailability, their clinical impact cannot be fully predicted without appropriately designed and conducted studies, such as those we report here.
When predicting the behavior of PAH-coated versus uncoated nanoparticles in biological systems, three different scenarios are possible. First, the reduction of the surface area could reduce the biological effects of CBNP, and differences in the PAHs would therefore not have an impact. Second, the PAH content could increase the biological effects of CBNP due to the toxicity of PAH – and so irrespective of the particle’s surface area. A third possibility is that toxicity could be decreased in some cell types due to the reduced surface area, but increased in other cell types due to PAH toxicity.
Biological effects of uncoated and PAH-coated CBNP
Supporting the hypothesis that a reduced surface area reduces the biological effects of CBNP [1, 10, 67], the coating of P90 with BaP prevented the P90-induced increase of the neutrophil attracting cytokine IL8 mRNA in A549 cells. As the high surface area of P90 is strongly associated with induction of oxidative stress, the reduced biological effect can be explained by the reduced ROS release that we observed with P90-BaP and P90-9NA in A549 cells compared to uncoated P90. This reduced biological effect is also supported by our observation that P90, and not P90-BaP, led to a transient increase in granulocyte influx in the in vivo experiment. This transient increase in granulocytes without changes of total cell number has previously been observed [68, 69]. The mechanism for the reduced number of macrophages is not known and would be an interesting area for future research. However, these results clearly demonstrate that surface area can be an important factor of CBNP effects in biological systems.
However, other results support the theory that PAH can increase the effects of CBNP . Firstly, we found that P90-BaP, P90-9NA and AS-PAH induced apoptosis in tracheal epithelial cells ex vivo, whereas P90 did not. This is consistent with previous reports describing apoptosis induction by PAH [54, 56, 57, 70]. Secondly, PAH-coated P90 and AS-PAH strongly induced Cyp mRNA expression. Cyp1a1 and Cyp1b1 mRNA are known to be induced by PAH in a subgroup of airway epithelial cells , and so their induction in both the trachea and intrapulmonary airways demonstrate that the PAH are biologically active. Interestingly, the assumed differences between P90-9NA, P90-BaP and AS-PAH are also reflected in the level of induction of Cyp1a1 and Cyp1b1 mRNA, in that P90-9NA resulted in the least induction, followed by P90-BaP, with the strongest induction by AS-PAH. Finally, AS-PAH was the only CBNP to reduce TEER in vitro, to increase BAL granulocyte content 14 days after the last application, and induce both histological changes in vivo and inflammatory mediator mRNA expression (KC and IL-6) ex vivo. Furthermore, AS-PAH had the highest apoptosis rate in tracheal epithelium ex vivo.
Our data therefore suggest that the overall biological impact of a PAH depends on the relative contribution of surface area reduction and PAH-induced toxicity, that is, in turn, influenced by the biological system. This is exemplified by cilia-driven particle transport speed, which is influenced by ciliary beat frequency, mucus release and epithelial cell death.
In the ex vivo trachea, P90 was the only particle to form aggregates. These aggregates bound to ciliated cells and increased ciliary beat frequency. Furthermore, microscopically visible nanoparticle agglomerates have been shown to increase ciliary beat frequency in the murine trachea . A similar effect has been observed in the bovine trachea where polystyrene particles >200 nm in diameter that attach to cilia increase ciliary beat frequency due to mechanical stimulation of axonema possibly involving second messengers such as cAMP or cGMP , both of which are known to increase ciliary beat frequency . In addition, mechanical stimulation of cilia triggers Ca2+ influx, with a consequent increase in ciliary beat frequency . Coating of P90 with PAHs prevented aggregate formation and consequently neither P90-BaP nor P90-9NA impact ciliary beat frequency. Thus, this shows that PAH coating of P90 reduced its effects on a biological system by changing its surface properties and not by a specific PAH toxicity. However, we detected an increase in epithelial cell apoptosis after application of P90-BaP and P90-9NA compared to P90. This increase in apoptosis together with the induction of Cyp enzymes can directly be ascribed to PAH toxicity .
AS-PAH increased ciliary beat frequency without forming microscopically visible agglomerates. Interestingly, fluoranthene, a PAH that we detected on the surface of AS-PAH, has previously been shown to induce a persistent increase in Ca2+ in Calu-3 cells, whereas BaP and anthracene did not . Since an increase in intracellular Ca2+ is known to increase ciliary beat frequency , it is possible that fluoranthene is directly responsible for the increased ciliary beat frequency. Despite increasing ciliary beat frequency there was a marked reduction in particle transport speed, possibly due to increased apoptosis and mucus release.
Previous research has shown that despite being bound to the surface of CBNP, PAHs can be bioavailable . Our data confirm that PAH bound to CBNP exerts biological effects in epithelial cells, as demonstrated by Cyp1a1 mRNA induction. Cyp1a1 mRNA expression is a very sensitive parameter for detecting biologically active PAHs . The induction of Cyp1a1 mRNA is triggered by binding of PAH to the aryl hydrocarbon receptor (AhR) that then translocates into the cell nucleus, and interacts with AhR nuclear translocator . This complex then induces Cyp1a1 mRNA expression by binding to xenobiotic response elements . In low concentrations, AhR activation suppresses the activity of the transcription factor p65 , which is known to induce the mRNA expression of IL-6 and IL-8 [81, 82]. We also observed a small but significant decrease in IL-6 mRNA expression in the epithelium of explanted tracheae, possibly due to AhR activation. At higher concentrations, PAHs can lead to IL-6 and IL-8 mRNA expression  – as we observed with AS-PAH. In general, AS-PAH had more effects than the PAH-coated CBNP, probably due to the composition of PAH present on the particle’s surface. As discussed above, the PAH fluoranthene induces prolonged increase in intracellular Ca2+ in Calu-3, whereas BaP and anthracene do not . Yamaguchi et al. also observed an increase of intracellular Ca2+ that was independent from AhR , indicating that PAH have additional activities apart from the classical AhR pathway. Identification of the specific PAH and/or PAH mixture that is responsible for the increased effects of AS-PAH would require additional research, although fluoranthene and compounds such as cyclopenta[cd]pyrene are possible candidates.
We have shown that AS-PAH exerts more toxic effects than P90 despite its reduced surface area. We attribute this to the composition of the surface PAHs. The relative toxicity of P90-9NA and P90-BaP, and the resultant biological effect, depended on the test system used.
Choice of test systems
In general, cell lines can give valuable information if the test substance is interfering with basal cell functions present in all cells. However, if cell lines lack specific pathways that are important for toxic effects, their value for predicting in vivo toxicity is limited .
The prediction of an in vivo effect based on results obtained in a single cell line is made more challenging, since both changes in surface area and direct PAH toxicity impact the overall effect of particles. This may explain why we had different results in terms of IL-8 mRNA production in different cell lines. The reason for these differences was most likely that some cell lines were more sensitive to the toxic effects of the PAHs, whereas others were more sensitive to changes in surface area. An in vivo experiment in which all target cell types are present is therefore in principle better suited to detect effects . However, in a single in vivo experiment, only a limited number of parameters can be determined. The readouts predetermined in the OECD experiment focus on inflammation and cell death. They do not address effects on mucociliary clearance. It should be noted, however that sustained impairment of mucociliary clearance can have substantial health effects [85–88] that are missed in animal models by focusing solely on inflammation and cell death. In principle, in vivo experiments can be designed to also include these parameters. However, this would be time consuming and cost intensive. The strength of short-term organ culture models is that they can be adapted to screen for specific organ functions that are not routinely examined in standardized in vivo experiments. This was demonstrated by using our ex vivo trachea model that allowed examining the complex interplay between mucus production, ciliary beating and cell death [72, 89].
Using a range of different test systems our results demonstrate that the biological effect of CBNP is determined by a combination of specific surface area and the composition of the surface-bound PAH, and varies in different target cells. Specifically, AS-PAH exerted more toxic effects than P90 despite its reduced surface area. We attribute this to the composition of the surface PAHs. However, the relative toxicity of P90-9NA and P90-BaP depended on the test system used.
The authors thank Sabine Bartel and Susanne Krauss-Etschmann (Borstel) for providing the 16HBE14o- cells for IL-8 mRNA expression experiments. Additionally, they thank all the participating patients and Dieter C. Gruenert for establishing the 16HBE14o- cell line, and Juliane Artelt (Borstel) and Gudrun Knebel (Lübeck) for expert technical assistance.
David Young, a professional medical writer from Young Medical Communications and Consulting Limited, critically reviewed and language-edited the manuscript prior to submission. This support was funded by the Borstel Research Center and the Universität zu Lübeck.
This work was funded by the Bundesministerium für Forschung und Bildung (03X0093).
Availability of data and materials
All data that are necessary to understand the theme of the study are included in this published article and its supplementary information files. Additional datasets generated or analyzed during the current study are available from corresponding author on reasonable request.
HF, PK and TH designed the study. MS and HB modified P90, produced the AS-PAH and characterized the CBNP. PG measured and analyzed the aerosol concentration and MMAD. EH performed and analyzed the histological analysis. CZ performed and analyzed the genotoxicological experiments. OC designed and supervised the rat inhalation study. GP was responsible for the generation and characterization of the exposure atmosphere. TT was the veterinarian responsible for the rat inhalation study. KL performed and analyzed the ex vivo studies on tracheal epithelial cells. CB, PK and GH designed the software for the ciliary beat frequency analysis. SS performed and analyzed the ex vivo studies on intrapulmonary bronchioles. TH and JK carried out and analyzed the in vitro experiments (ROS, TEER). IL-8 mRNA expression of 16HBE14o- and Calu-3 cells was analyzed by SW and AJ. AJ determined the IL-6 and IL-8 protein release from Calu-3 cells. JK characterized the CBNP in water and media. KL and PK drafted the manuscript. All authors contributed to the intellectual content of the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
The study was performed in accordance with the German animal protection law and approved by the appropriate governmental authorities.
The OECD inhalation study on rats was approved by the Niedersächsisches Landesamt für Verbraucherschutz und Lebensmittelsicherheit. The ex vivo studies on mouse trachea and intrapulmonary bronchioles were approved by the Ministerium für Landwirtschaft, Umwelt und ländliche Räume des Landes Schleswig-Holstein.
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- Stoeger T, Reinhard C, Takenaka S, Schroeppel A, Karg E, Ritter B, et al. Instillation of six different ultrafine carbon particles indicates a surface area threshold dose for acute lung inflammation in mice. Environ Health Perspect. 2006;114:328–33.View ArticlePubMedGoogle Scholar
- Sahu D, Kannan GM, Vijayaraghavan R. Carbon black particle exhibits size dependent toxicity in human monocytes. Int J Inflam. 2014;2014:1–10.View ArticleGoogle Scholar
- Marzaioli V, Aguilar-Pimentel JA, Weichenmeier I, Luxenhofer G, Wiemann M, Landsiedel R, et al. Surface modifications of silica nanoparticles are crucial for their inert versus proinflammatory and immunomodulatory properties. Int J Nanomedicine. 2014;9:2815–32.PubMedPubMed CentralGoogle Scholar
- Cheng WJ, Rong Y, Shi TM, Zhou T, Liu YW, Chen WH. Size-dependent biological effects on vascular endothelial cells induced by different particulate matters. J Huazhong Univ Sci Technolog Med Sci. 2014;34:314–21.View ArticlePubMedGoogle Scholar
- Braakhuis HM, Park MV, Gosens I, De Jong WH, Cassee FR. Physicochemical characteristics of nanomaterials that affect pulmonary inflammation. Part Fibre Toxicol. 2014;11:18.View ArticlePubMedPubMed CentralGoogle Scholar
- Ma JYC, Ma JKH. The dual effect of the particulate and organic components of diesel exhaust particles on the alteration of pulmonary immune/inflammatory responses and metabolic enzymes. J Environ Sci Health C. 2002;20:117–47.View ArticleGoogle Scholar
- Gatoo MA, Naseem S, Arfat MY, Dar AM, Qasim K, Zubair S. Physicochemical properties of nanomaterials: implication in associated toxic manifestations. Biomed Res Int. 2014;2014:498420.View ArticlePubMedPubMed CentralGoogle Scholar
- Shin SW, Song IH, Um SH. Role of physicochemical properties in nanoparticle toxicity. Nanomaterials. 2015;5:1351–65.View ArticleGoogle Scholar
- Stoeger T, Schmid O, Takenaka S, Schulz H. Inflammatory response to TiO2 and carbonaceous particles scales best with BET surface area. Environ Health Perspect. 2007;115:A290–1.View ArticlePubMedPubMed CentralGoogle Scholar
- Schmid O, Stoeger T. Surface area is the biologically most effective dose metric for acute nanoparticle toxicity in the lung. J Aerosol Sci. 2016;99:133–43.View ArticleGoogle Scholar
- Hussain S, Boland S, Baeza-Squiban A, Hamel R, Thomassen LC, Martens JA, et al. Oxidative stress and proinflammatory effects of carbon black and titanium dioxide nanoparticles: role of particle surface area and internalized amount. Toxicology. 2009;260:142–9.View ArticlePubMedGoogle Scholar
- Vesterdal LK, Mikkelsen L, Folkmann JK, Sheykhzade M, Cao Y, Roursgaard M, et al. Carbon black nanoparticles and vascular dysfunction in cultured endothelial cells and artery segments. Toxicol Lett. 2012;214:19–26.View ArticlePubMedGoogle Scholar
- Ma-Hock L, Strauss V, Treumann S, Kuttler K, Wohlleben W, Hofmann T, et al. Comparative inhalation toxicity of multi-wall carbon nanotubes, graphene, graphite nanoplatelets and low surface carbon black. Part Fibre Toxicol. 2013;10:23.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang R, Dai Y, Zhang X, Niu Y, Meng T, Li Y, et al. Reduced pulmonary function and increased pro-inflammatory cytokines in nanoscale carbon black-exposed workers. Part Fibre Toxicol. 2014;11:73.View ArticlePubMedPubMed CentralGoogle Scholar
- Boström CE, Gerde P, Hanberg A, Jernstrom B, Johansson C, Kyrklund T, et al. Cancer risk assessment, indicators, and guidelines for polycyclic aromatic hydrocarbons in the ambient air. Environ Health Perspect. 2002;110:451–88.View ArticlePubMedPubMed CentralGoogle Scholar
- Kong HT, Xia K, Pan L, Zhang JC, Luo Y, Zhang Y, et al. Autophagy and lysosomal dysfunction: a new insight into mechanism of synergistic pulmonary toxicity of carbon black-metal ions co-exposure. Carbon. 2017;111:322–33.View ArticleGoogle Scholar
- Valavanidis A, Fiotakis K, Vlachogianni T. Airborne particulate matter and human health: toxicological assessment and importance of size and composition of particles for oxidative damage and carcinogenic mechanisms. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2008;26:339–62.View ArticlePubMedGoogle Scholar
- Lighty JS, Veranth JM, Sarofim AF. Combustion aerosols: factors governing their size and composition and implications to human health. J Air Waste Manag Assoc. 2000;50:1565–618.View ArticlePubMedGoogle Scholar
- Oberdörster G, Oberdörster E, Oberdörster J. Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles. Environ Health Perspect. 2005;113:823–39.View ArticlePubMedPubMed CentralGoogle Scholar
- Bonvallot V, Baeza-Squiban A, Baulig A, Brulant S, Boland S, Muzeau F, et al. Organic compounds from diesel exhaust particles elicit a proinflammatory response in human airway epithelial cells and induce cytochrome p450 1A1 expression. Am J Respir Cell Mol Biol. 2001;25:515–21.View ArticlePubMedGoogle Scholar
- World Health Organization. Air quality guidelines for Europe. WHO Reg Publ Eur Ser. 2000;V-X:1–273.Google Scholar
- Shimada T, Fujii-Kuriyama Y. Metabolic activation of polycyclic aromatic hydrocarbons to carcinogens by cytochromes P450 1A1 and 1B1. Cancer Sci. 2004;95:1–6.View ArticlePubMedGoogle Scholar
- Baird WM, Hooven LA, Mahadevan B. Carcinogenic polycyclic aromatic hydrocarbon-DNA adducts and mechanism of action. Environ Mol Mutagen. 2005;45:106–14.View ArticlePubMedGoogle Scholar
- Dasenbrock C, Peters L, Creutzenberg O, Heinrich U. The carcinogenic potency of carbon particles with and without PAH after repeated intratracheal administration in the rat. Toxicol Lett. 1996;88:15–21.View ArticlePubMedGoogle Scholar
- Goulaouic S, Foucaud L, Bennasroune A, Laval-Gilly P, Falla J. Effect of polycyclic aromatic hydrocarbons and carbon black particles on pro-inflammatory cytokine secretion: impact of PAH coating onto particles. J Immunotoxicol. 2008;5:337–45.View ArticlePubMedGoogle Scholar
- Mroz RM, Schins RP, Li H, Drost EM, Macnee W, Donaldson K. Nanoparticle carbon black driven DNA damage induces growth arrest and AP-1 and NFkappaB DNA binding in lung epithelial A549 cell line. J Physiol Pharmacol. 2007;58 Suppl 5:461–70.PubMedGoogle Scholar
- Mroz RM, Schins RP, Li H, Jimenez LA, Drost EM, Holownia A, et al. Nanoparticle-driven DNA damage mimics irradiation-related carcinogenesis pathways. Eur Respir J. 2008;31:241–51.View ArticlePubMedGoogle Scholar
- Borm PJA, Cakmak G, Jermann E, Weishaupt C, Kempers P, van Schooten FJ, et al. Formation of PAH-DNA adducts after in vivo and vitro exposure of rats and lung cells to different commercial carbon blacks. Toxicol Appl Pharmacol. 2005;205:157–67.View ArticlePubMedGoogle Scholar
- Chin BY, Choi ME, Burdick MD, Strieter RM, Risby TH, Choi AMK. Induction of apoptosis by particulate matter: role of TNF-alpha and MAPK. Am J Physiol Lung Cell Mol Physiol. 1998;275:L942–9.Google Scholar
- Delfino RJ. Epidemiologic evidence for asthma and exposure to air toxics: linkages between occupational, indoor, and community air pollution research. Environ Health Perspect. 2002;110 Suppl 4:573–89.PubMedPubMed CentralGoogle Scholar
- Balmes JR. How does diesel exhaust impact asthma? Thorax. 2011;66:4–6.View ArticlePubMedGoogle Scholar
- OECD. Guideline for Testing of Chemicals, Test Guideline 412. Repeated Dose Inhalation Toxicity: 28-day or 14-day Study. 1981; http://www.nikkakyo.org/ontai/merumaga/Challenge/OECD/TG_412.pdf. Accessed on 21th December 2016.
- Sager TM, Castranova V. Surface area of particle administered versus mass in determining the pulmonary toxicity of ultrafine and fine carbon black: comparison to ultrafine titanium dioxide. Part Fibre Toxicol. 2009;6:15.View ArticlePubMedPubMed CentralGoogle Scholar
- Landsiedel R, Ma-Hock L, Kroll A, Hahn D, Schnekenburger J, Wiench K, et al. Testing metal-oxide nanomaterials for human safety. Adv Mater. 2010;22:2601–27.View ArticlePubMedGoogle Scholar
- Verma N, Pink M, Rettenmeier AW, Schmitz-Spanke S. Review on proteomic analyses of benzo[a]pyrene toxicity. Proteomics. 2012;12:1731–55.View ArticlePubMedGoogle Scholar
- Boysen G, Hecht SS. Analysis of DNA and protein adducts of benzo[a]pyrene in human tissues using structure-specific methods. Mutat Res. 2003;543:17–30.View ArticlePubMedGoogle Scholar
- Shen YM, Troxel AB, Vedantam S, Penning TM, Field J. Comparison of p53 mutations induced by PAH o-quinones with those caused by anti-benzo[a]pyrene diol epoxide in vitro: role of reactive oxygen and biological selection. Chem Res Toxicol. 2006;19:1441–50.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang LJ, Bao Y, Li J. Nuclear respiratory factor-1 is involved in mitochondrial dysfunction induced by benzo(a)pyrene in human bronchial epithelial cells. Basic Clin Pharmacol Toxicol. 2011;109:115–22.View ArticlePubMedGoogle Scholar
- Ovrevik J, Arlt VM, Oya E, Nagy E, Mollerup S, Phillips DH, et al. Differential effects of nitro-PAHs and amino-PAHs on cytokine and chemokine responses in human bronchial epithelial BEAS-2B cells. Toxicol Appl Pharmacol. 2010;242:270–80.View ArticlePubMedGoogle Scholar
- Harrigan JA, McGarrigle BP, Sutter TR, Olson JR. Tissue specific induction of cytochrome P450 (CYP) 1A1 and 1B1 in rat liver and lung following in vitro (tissue slice) and in vivo exposure to benzo(a)pyrene. Toxicol In Vitro. 2006;20:426–38.View ArticlePubMedGoogle Scholar
- Fu PP, Von Tungeln LS, Chou MW. Metabolism of 9-nitroanthracene by rat liver microsomes: identification and mutagenicity of metabolites. Carcinogenesis. 1985;6:753–7.View ArticlePubMedGoogle Scholar
- Fu PP, Heflich RH, Von Tungeln LS, Yang DT, Fifer EK, Beland FA. Effect of the nitro group conformation on the rat liver microsomal metabolism and bacterial mutagenicity of 2- and 9-nitroanthracene. Carcinogenesis. 1986;7:1819–27.View ArticlePubMedGoogle Scholar
- Durant JL, Busby Jr WF, Lafleur AL, Penman BW, Crespi CL. Human cell mutagenicity of oxygenated, nitrated and unsubstituted polycyclic aromatic hydrocarbons associated with urban aerosols. Mutat Res. 1996;371:123–57.View ArticlePubMedGoogle Scholar
- Stroebele MR, Bockhorn H. Effects of synthesis conditions on the content of polycyclic aromatic hydrocarbons on the surface of carbon black nanoparticles. Proceedings of the European Combustion Meeting. 2013:Paper P5-52.Google Scholar
- Cozens AL, Yezzi MJ, Kunzelmann K, Ohrui T, Chin L, Eng K, et al. CFTR expression and chloride secretion in polarized immortal human bronchial epithelial cells. Am J Respir Cell Mol Biol. 1994;10:38–47.View ArticlePubMedGoogle Scholar
- Andersen CL, Jensen JL, Orntoft TF. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 2004;64:5245–50.View ArticlePubMedGoogle Scholar
- Zehethofer N, Bermbach S, Hagner S, Garn H, Muller J, Goldmann T, et al. Lipid analysis of airway epithelial cells for studying respiratory diseases. Chromatographia. 2015;78:403–13.View ArticlePubMedGoogle Scholar
- Crouzier T, Beckwitt CH, Ribbeck K. Mucin multilayers assembled through sugar-lectin interactions. Biomacromolecules. 2012;13:3401–8.View ArticlePubMedGoogle Scholar
- Kawano K, Uehara F, Sameshima M, Ohba N. Application of lectins for detection of goblet cell carbohydrates of the human conjunctiva. Exp Eye Res. 1984;38:439–47.View ArticlePubMedGoogle Scholar
- Chazotte B. Labeling membrane glycoproteins or glycolipids with fluorescent wheat germ agglutinin. Cold Spring Harb Protoc. 2011;2011:pdb prot5623.Google Scholar
- Knaapen AM, Borm PJ, Albrecht C, Schins RP. Inhaled particles and lung cancer. Part A: mechanisms. Int J Cancer. 2004;109:799–809.View ArticlePubMedGoogle Scholar
- Yang H, Liu C, Yang D, Zhang H, Xi Z. Comparative study of cytotoxicity, oxidative stress and genotoxicity induced by four typical nanomaterials: the role of particle size, shape and composition. J Appl Toxicol. 2009;29:69–78.View ArticlePubMedGoogle Scholar
- Podechard N, Lecureur V, Le Ferrec E, Guenon I, Sparfel L, Gilot D, et al. Interleukin-8 induction by the environmental contaminant benzo(a)pyrene is aryl hydrocarbon receptor-dependent and leads to lung inflammation. Toxicol Lett. 2008;177:130–7.View ArticlePubMedGoogle Scholar
- Jyonouchi H. Airway epithelium and apoptosis. Apoptosis. 1999;4:407–17.View ArticlePubMedGoogle Scholar
- Kim KB, Lee BM. Oxidative stress to DNA, protein, and antioxidant enzymes (superoxide dismutase and catalase) in rats treated with benzo(a)pyrene. Cancer Lett. 1997;113:205–12.View ArticlePubMedGoogle Scholar
- Tesfaigzi Y. Roles of apoptosis in airway epithelia. Am J Respir Cell Mol Biol. 2006;34:537–47.View ArticlePubMedPubMed CentralGoogle Scholar
- Revel A, Raanani H, Younglai E, Xu J, Rogers I, Han R, et al. Resveratrol, a natural aryl hydrocarbon receptor antagonist, protects lung from DNA damage and apoptosis caused by benzo[a]pyrene. J Appl Toxicol. 2003;23:255–61.View ArticlePubMedGoogle Scholar
- Tsuji PA, Walle T. Inhibition of benzo[a]pyrene-activating enzymes and DNA binding in human bronchial epithelial BEAS-2B cells by methoxylated flavonoids. Carcinogenesis. 2006;27:1579–85.View ArticlePubMedGoogle Scholar
- Elovaara E, Mikkola J, Stockmann-Juvala H, Luukkanen L, Keski-Hynnila H, Kostiainen R, et al. Polycyclic aromatic hydrocarbon (PAH) metabolizing enzyme activities in human lung, and their inducibility by exposure to naphthalene, phenanthrene, pyrene, chrysene, and benzo(a)pyrene as shown in the rat lung and liver. Arch Toxicol. 2007;81:169–82.View ArticlePubMedGoogle Scholar
- Donnet J-B, Bansal RC, Wang M-J. Carbon black - science and technology. Secondth ed. New York - Basel: Marcel Dekker, Inc; 1993.Google Scholar
- Schreiber N, Strobele M, Kopf J, Hochscheid R, Kotte E, Weber P, et al. Lung alterations following single or multiple low-dose carbon black nanoparticle aspirations in mice. J Toxicol Environ Health A. 2013;76:1317–32.View ArticlePubMedGoogle Scholar
- Schreiber N, Strobele M, Hochscheid R, Kotte E, Weber P, Bockhorn H, et al. Modifications of carbon black nanoparticle surfaces modulate type II pneumocyte homoeostasis. J Toxicol Environ Health A. 2016;79:153–64.View ArticlePubMedGoogle Scholar
- Moorthy B, Chu C, Carlin DJ. Polycyclic aromatic hydrocarbons: from metabolism to lung cancer. Toxicol Sci. 2015;145:5–15.View ArticlePubMedPubMed CentralGoogle Scholar
- Keohavong P, Melacrinos A, Shukla R. In vitro mutational spectrum of cyclopenta[cd]pyrene in the human HPRT gene. Carcinogenesis. 1995;16:855–60.View ArticlePubMedGoogle Scholar
- Nesnow S, Mass MJ, Ross JA, Galati AJ, Lambert GR, Gennings C, et al. Lung tumorigenic interactions in strain A/J mice of five environmental polycyclic aromatic hydrocarbons. Environ Health Perspect. 1998;106 Suppl 6:1337–46.View ArticlePubMedPubMed CentralGoogle Scholar
- Gibb HJ, Chen CW. Multistage model interpretation of additive and multiplicative carcinogenic effects. Risk Anal. 1986;6:167–70.View ArticlePubMedGoogle Scholar
- Monteiller C, Tran L, MacNee W, Faux S, Jones A, Miller B, et al. The pro-inflammatory effects of low-toxicity low-solubility particles, nanoparticles and fine particles, on epithelial cells in vitro: the role of surface area. Occup Environ Med. 2007;64:609–15.View ArticlePubMedPubMed CentralGoogle Scholar
- Renwick LC, Brown D, Clouter A, Donaldson K. Increased inflammation and altered macrophage chemotactic responses caused by two ultrafine particle types. Occup Environ Med. 2004;61:442–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Kyjovska ZO, Jacobsen NR, Saber AT, Bengtson S, Jackson P, Wallin H, et al. DNA damage following pulmonary exposure by instillation to low doses of carbon black (Printex 90) nanoparticles in mice. Environ Mol Mutagen. 2015;56:41–9.View ArticlePubMedGoogle Scholar
- Solhaug A, Refsnes M, Lag M, Schwarze PE, Husoy T, Holme JA. Polycyclic aromatic hydrocarbons induce both apoptotic and anti-apoptotic signals in Hepa1c1c7 cells. Carcinogenesis. 2004;25:809–19.View ArticlePubMedGoogle Scholar
- Chang H, Chang LW, Cheng YH, Tsai WT, Tsai MX, Lin PP. Preferential induction of CYP1A1 and CYP1B1 in CCSP-positive cells. Toxicol Sci. 2006;89:205–13.View ArticlePubMedGoogle Scholar
- Bermbach S, Weinhold K, Roeder T, Petersen F, Kugler C, Goldmann T, et al. Mechanisms of cilia-driven transport in the airways in the absence of mucus. Am J Respir Cell Mol Biol. 2014;51:56–67.View ArticlePubMedGoogle Scholar
- Navarrette CR, Sisson JH, Nance E, Allen-Gipson D, Hanes J, Wyatt TA. Particulate matter in cigarette smoke increases ciliary axoneme beating through mechanical stimulation. J Aerosol Med Pulm Drug Deliv. 2012;25:159–68.View ArticlePubMedPubMed CentralGoogle Scholar
- Salathe M. Regulation of mammalian ciliary beating. Annu Rev Physiol. 2007;69:401–22.View ArticlePubMedGoogle Scholar
- Li WE, Chen W, Ma YF, Tuo QR, Luo XJ, Zhang T, et al. Methods to measure and analyze ciliary beat activity: Ca2+ influx-mediated cilia mechanosensitivity. Pflugers Arch. 2012;464:671–80.View ArticlePubMedGoogle Scholar
- Ito Y, Son M, Sato S, Ohashi T, Kondo M, Shimokata K, et al. Effects of fluoranthene, a polycyclic aromatic hydrocarbon, on cAMP-dependent anion secretion in human airway epithelia. J Pharmacol Exp Ther. 2004;308:651–7.View ArticlePubMedGoogle Scholar
- Gerde P, Muggenburg BA, Lundborg M, Dahl AR. The rapid alveolar absorption of diesel soot-adsorbed benzo[a]pyrene: bioavailability, metabolism and dosimetry of an inhaled particle-borne carcinogen. Carcinogenesis. 2001;22:741–9.View ArticlePubMedGoogle Scholar
- Totlandsdal AI, Cassee FR, Schwarze P, Refsnes M, Lag M. Diesel exhaust particles induce CYP1A1 and pro-inflammatory responses via differential pathways in human bronchial epithelial cells. Part Fibre Toxicol. 2010;7:41.View ArticlePubMedPubMed CentralGoogle Scholar
- Rowlands JC, Gustafsson JA. Aryl hydrocarbon receptor-mediated signal transduction. Crit Rev Toxicol. 1997;27:109–34.View ArticlePubMedGoogle Scholar
- Ovrevik J, Lag M, Lecureur V, Gilot D, Lagadic-Gossmann D, Refsnes M, et al. AhR and Arnt differentially regulate NF-kappaB signaling and chemokine responses in human bronchial epithelial cells. Cell Commun Signal. 2014;12:48.View ArticlePubMedPubMed CentralGoogle Scholar
- Kunsch C, Rosen CA. NF-kappa B subunit-specific regulation of the interleukin-8 promoter. Mol Cell Biol. 1993;13:6137–46.View ArticlePubMedPubMed CentralGoogle Scholar
- Libermann TA, Baltimore D. Activation of interleukin-6 gene-expression through the NF-kappa B transcription factor. Mol Cell Biol. 1990;10:2327–34.View ArticlePubMedPubMed CentralGoogle Scholar
- Yamaguchi K, Near R, Shneider A, Cui HL, Ju ST, Sherr DH. Fluoranthene-induced apoptosis in murine T cell hybridomas is independent of the aromatic hydrocarbon receptor. Toxicol Appl Pharmacol. 1996;139:144–52.View ArticlePubMedGoogle Scholar
- Saeidnia S, Manayi A, Abdollahi M. From in vitro experiments to in vivo and clinical studies; pros and cons. Curr Drug Discov Technol. 2015;12:218–24.View ArticlePubMedGoogle Scholar
- Munkholm M, Mortensen J. Mucociliary clearance: pathophysiological aspects. Clin Physiol Funct Imaging. 2014;34:171–7.View ArticlePubMedGoogle Scholar
- Hoegger MJ, Fischer AJ, McMenimen JD, Ostedgaard LS, Tucker AJ, Awadalla MA, et al. Impaired mucus detachment disrupts mucociliary transport in a piglet model of cystic fibrosis. Science. 2014;345:818–22.View ArticlePubMedPubMed CentralGoogle Scholar
- Livraghi A, Randell SH. Cystic fibrosis and other respiratory diseases of impaired mucus clearance. Toxicol Pathol. 2007;35:116–29.View ArticlePubMedGoogle Scholar
- D'Amato G. Environmental urban factors (air pollution and allergens) and the rising trends in allergic respiratory diseases. Allergy. 2002;57 Suppl 72:30–3.View ArticlePubMedGoogle Scholar
- König P, Krain B, Krasteva G, Kummer W. Serotonin increases cilia-driven particle transport via an acetylcholine-independent pathway in the mouse trachea. Plos One. 2009;4:e4938.View ArticlePubMedPubMed CentralGoogle Scholar
- International Agency for Research on Cancer. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. http://monographs.iarc.fr/ENG/Classification/. Accessed on 16th November 2016.